Seismic wave amplitudes have tremendous sensitivity to subduction structure; however, they are affected by attenuation, scattering and focusing, and have therefore been sparsely used compared with traveltimes. We measure and model teleseismic body wave amplitudes recorded at a dense broadband array in the Washington Cascades. These data show anomalous amplitude variations with complex azimuthal dependence at frequencies as low as 0.05 Hz, accompanied by significant multipathing. We demonstrate using spectral-element numerical simulations that focusing of the teleseismic wavefield by the Juan de Fuca slab is responsible for some of the amplitude anomalies. The focusing effects can contaminate the apparent differential attenuation measurements and produce at least 20% of the inferred attenuation signal. Our results indicate that the amplitudes are sensitive to the subducting slab geometry and subduction structure, and can be used to refine seismic images. Ubiquitous and consistent amplitude anomalies are observed along the arc, suggesting that the Juan de Fuca slab may be continuous from Canada to northern California.

alpha- and beta-tubulin form heterodimers, with GTPase activity, that assemble into microtubules. Like other GTPases, the nucleotide-bound state of tubulin heterodimers controls whether the molecules are in a biologically active or inactive state. While alpha-tubulin in the heterodimer is constitutively bound to GTP, beta-tubulin can be bound to either GDP (GDP-tubulin) or GTP (GTP-tubulin). GTP-tubulin hydrolyzes its GTP to GDP following assembly into a microtubule and, upon disassembly, must exchange its bound GDP for GTP to participate in subsequent microtubule polymerization. Tubulin dimers have been shown to exhibit rapid intrinsic nucleotide exchange in vitro, leading to a commonly accepted belief that a tubulin guanine nucleotide exchange factor (GEF) may be unnecessary in cells. Here, we use quantitative binding assays to show that BuGZ, a spindle assembly factor, binds tightly to GDP-tubulin, less tightly to GTP-tubulin, and weakly to microtubules. We further show that BuGZ promotes the incorporation of GTP into tubulin using a nucleotide exchange assay. The discovery of a tubulin GEF suggests a mechanism that may aid rapid microtubule assembly dynamics in cells.

In continuation of our comprehensive study of the carbonaceous chondrites, we here present data for the CM chondrites. Our study's aim is to determine the abundances and the average elemental compositions of the major and minor chondritic components. The overarching goal has been to explore the fundamental question of whether chondrules and matrix are complementary, i.e., genetically related, or if these major chondritic components evolved separately before accretion as in the four-component model.

We clarify the nature of hafnia as a proper ferroelectric and show that there is a shallow double well involving a single soft polar mode as in well-known classic ferroelectrics. Using symmetry analysis, density functional theory structural optimizations with and without epitaxial strain, and density functional perturbation theory, we examine several important possible hafnia structures derived ultimately from the cubic fluorite structure, including baddeleyite (P21/c), tetragonal antiferroelectric P42nmc, Pbca (nonpolar and brookite), ferroelectric rhombohedral (R3m and R3), Pmn21, and Pca21 structures. The latter is considered to be the most likely ferroelectric phase seen experimentally and has an antiferroelectric parent with space group Pbcn, with a single unstable polar mode and a shallow double well with a well depth of 24 meV/atom. Strain is not required for switching or other ferroelectric properties, nor is coupling of the soft mode with any other modes within the ferroelectric Pca21, Pmn21, R3m, or R3 phases.