The search for definitive biosignatures-unambiguous markers of past or present life-is a central goal of paleobiology and astrobiology. We used pyrolysis-gas chromatography coupled to mass spectrometry to analyze chemically disparate samples, including living cells, geologically processed fossil organic material, carbon-rich meteorites, and laboratory-synthesized organic compounds and mixtures. Data from each sample were employed as training and test subsets for machine-learning methods, which resulted in a model that can identify the biogenicity of both contemporary and ancient geologically processed samples with ~90% accuracy. These machine-learning methods do not rely on precise compound identification: Rather, the relational aspects of chromatographic and mass peaks provide the needed information, which underscores this method's utility for detecting alien biology.

The origin of life on earth requires the synthesis of protobiopolymers in realistic geologic environments along strictly abiotic pathways that rely on inorganic phases (such as minerals) instead of cellular machinery to promote condensation. One such class of polymer central to biochemistry is the polynucleotides, and oligomerization of activated ribonucleotides has been widely studied. Nonetheless, the range of laboratory conditions tested to date is limited and the impact of realistic early Earth conditions on condensation reactions remains unexplored. Here, we investigate the potential for a variety of minerals to enhance oligomerization using ribonucleotide monomers as one example to model condensation under plausible planetary conditions. The results show that several minerals differing in both structure and composition enhance oligomerization. Sulfide minerals yielded oligomers of comparable lengths to those formed in the presence of clays, with galena being the most effective, yielding oligonucleotides up to six bases long. Montmorillonite continues to excel beyond other clays. Chemical pretreatment of the clay was not required, though maximum oligomer lengths decreased from ~11 to 6 bases. These results demonstrate the diversity of mineral phases that can impact condensation reactions and highlight the need for greater consideration of environmental context when assessing prebiotic synthesis and the origin of life.

Metallicity gradients refer to the sloped radial profiles of the metallicities of gas and stars and are commonly seen in disk galaxies. A well-defined metallicity gradient of the Galactic disk is observed particularly well with classical Cepheids, which are good stellar tracers thanks to their period-luminosity relation, allowing precise distance estimation and other advantages. However, the measurement of the inner-disk gradient has been impeded by the incompleteness of previous samples of Cepheids and the limitations of optical spectroscopy in observing highly reddened objects. Here we report the metallicities of 16 Cepheids measured with high-resolution spectra in the near-infrared YJ bands. These Cepheids are located at 3-5.6 kpc in Galactocentric distance, R-GC, and reveal the metallicity gradient in this range for the first time. Their metallicities are mostly between 0.1 and 0.3 dex in [Fe/H] and more or less follow the extrapolation of the metallicity gradient found in the outer part, R-GC > 6.5 kpc. The gradient in the inner disk may be shallower or even flat, but the small sample does not allow the determination of the slope precisely. More extensive spectroscopic observations would also be necessary for studying minor populations, if any, with higher or lower metallicities that were reported in previous literature. In addition, the 3D velocities of our inner-disk Cepheids show a kinematic pattern that indicates noncircular orbits caused by the Galactic bar, which is consistent with the patterns reported in recent studies on high-mass star-forming regions and red giant branch stars.

Stage 55, T3 exposure for 12 hours.

Stage 55, T3 exposure for 48 hours.

Stage 55, T3 exposure for 48 hours.

adult frog no T3 treatment.

stage 55 control samples, No T3 Exposure.

Stage 55, T3 exposure for 24 hours.