The development of multi-cellular organisms requires coordinated changes in gene expression that are often mediated by the interaction between transcription factors (TFs) and their corresponding cis-regulatory elements (CREs). During development and differentiation, the accessibility of CREs is dynamically modulated by the epigenome. How the epigenome, CREs and TFs together exert control over cell fate commitment remains to be fully understood. In the Arabidopsis leaf epidermis, meristemoids undergo a series of stereotyped cell divisions, then switch fate to commit to stomatal differentiation. Newly created or reanalyzed scRNA-seq and ChIP-seq data confirm that stomatal development involves distinctive phases of transcriptional regulation and that differentially regulated genes are bound by the stomatal basic-helix-loop-helix (bHLH) TFs. Targets of the bHLHs often reside in repressive chromatin before activation. MNase-seq evidence further suggests that the repressive state can be overcome and remodeled upon activation by specific stomatal bHLHs. We propose that chromatin remodeling is mediated through the recruitment of a set of physical interactors that we identified through proximity labeling - the ATPase-dependent chromatin remodeling SWI/SNF complex and the histone acetyltransferase HAC1. The bHLHs and chromatin remodelers localize to overlapping genomic regions in a hierarchical order. Furthermore, plants with stage-specific knock-down of the SWI/SNF components or HAC1 fail to activate specific bHLH targets and display stomatal development defects. Together these data converge on a model for how stomatal TFs and epigenetic machinery cooperatively regulate transcription and chromatin remodeling during progressive fate specification.

Protein-protein interactions play a crucial role in driving cellular processes and enabling appropriate physiological responses in organisms. The plant hormone ethylene signaling pathway is complex and regulated by the spatiotemporal regulation of its signaling molecules. Constitutive Triple Response 1 (CTR1), a key negative regulator of the pathway, regulates the function of Ethylene-Insensitive 2 (EIN2), a positive regulator of ethylene signaling, at the endoplasmic reticulum (ER) through phosphorylation. Our recent study revealed that CTR1 can also translocate from the ER to the nucleus in response to ethylene and positively regulate ethylene responses by stabilizing EIN3. To gain further insights into the role of CTR1 in plants, we used TurboID-based proximity labeling and mass spectrometry to identify the proximal proteomes of CTR1 in Nicotiana benthamiana. The identified proximal proteins include known ethylene signaling components, as well as proteins involved in diverse cellular processes such as mitochondrial respiration, mRNA metabolism, and organelle biogenesis. Our study demonstrates the feasibility of proximity labeling using the N. benthamiana transient expression system and identifies the potential interactors of CTR1 in vivo, uncovering the potential roles of CTR1 in a wide range of cellular processes.

Recent discoveries of gas giant exoplanets around M-dwarfs from transiting and radial velocity surveys are difficult to explain with core-accretion models. We present here a homogeneous suite of 162 models of gravitationally unstable gaseous disks. These models represent an existence proof for gas giants more massive than 0.1 Jupiter masses to form by the gas disk gravitational instability (GDGI) mechanism around M-dwarfs for comparison with observed exoplanet demographics and protoplanetary disk mass estimates for M-dwarf stars. We use the Enzo 2.6 adaptive mesh refinement (AMR) 3D hydrodynamics code to follow the formation and initial orbital evolution of gas giant protoplanets in gravitationally unstable gaseous disks in orbit around M-dwarfs with stellar masses ranging from 0.1 M circle dot to 0.5 M circle dot. The gas disk masses are varied over a range from disks that are too low in mass to form gas giants rapidly to those where numerous gas giants are formed, therefore revealing the critical disk mass necessary for gas giants to form by the GDGI mechanism around M-dwarfs. The disk masses vary from 0.01 M circle dot to 0.05 M circle dot while the disk to star mass ratios explored the range from 0.04 to 0.3. The models have varied initial outer disk temperatures (10-60 K) and varied levels of AMR grid spatial resolution, producing a sample of expected gas giant protoplanets for each star mass. Broadly speaking, disk masses of at least 0.02 M circle dot are needed for the GDGI mechanism to form gas giant protoplanets around M-dwarfs.

Mantle xenoliths in a Mesoproterozoic lamprophyre dyke at Elliot Lake, Ontario, located on the east margin of the Midcontinent Rift (MCR), erupted at similar to 1.1 Ga. These xenoliths enable a study of critical metal enrichment in the sub-cratonic lithospheric mantle (SCLM). Whole-rock major and trace element data from a suite of peridotite xenoliths document a combination of melt depletion and cryptic metasomatic processes. Trace element whole-rock and mineral systematics show a specific endowment in Nb-U-REE (ca. 5-30 ppm mean value), linked to carbonated silicate metasomatism. Geochronological data from the lamprophyre host (Rb-Sr age of 1112.8 +/- 4.95 Ma) and the mantle xenoliths (Re-Os) indicate that our samples document the state of the mantle during the earlier stages of magmatism of the MCR. Mineral thermobarometry reveals a hot geotherm reflecting the thinning of the Superior cratonic root to 110 km. Most of the Nb-U-REE deposits and anomalies associated with the MCR event are located around Lake Superior. Here we document for the first time north of Lake Huron, metasomatic processes in the lithosphere that may have created Nb-U-REE metal endowment. The mantle events documented here relate to other observations made in the Slave and North China craton and show how silico-carbonated mid-lithospheric metasomatism up-grades the cratonic lithospheric mantle into a fertile source. Comparison with other small degree melts such as kimberlites, and mantle metasomes related to the MARID suite, show that small degree melts are very efficient at transporting critical metals from the HFSE group plus U and Th, into Earth's lithosphere.

The mineral kingdom has experienced dramatic increases in diversity and complexity through billions of years of planetary evolution as a consequence of a sequence of physical, chemical, and biological processes. Each new formational environment, or "mineral paragenetic mode," has its own characteristic attributes, including the stage of mineral evolution and geological age, ranges of T, P, duration of formation events, and other environmental influences on mineral formation. Furthermore, the minerals associated with each paragenetic mode have a wide range of average properties, including hardness, density, and chemical and structural complexity. A survey of attributes of 57 mineral paragenetic modes representing the full range of mineral-forming processes reveals systematic trends, including: (1) minerals documented from older paragenetic processes are systematically harder on average than those from more recent processes; (2) minerals from paragenetic modes formed at lower T (notably <500 K) display greater average structural complexity than those formed at high T (especially >1000 K); and (3) minerals from paragenetic modes that display greater average chemical complexity are systematically less dense than those from modes with lesser average chemical complexity. In addition, minerals formed in anhydrous environments and/or by abiotic processes are, on average, significantly denser and harder than those formed in hydrous environments and/or by biotic processes.

Part VII of the evolutionary system of mineralogy catalogs, analyzes, and visualizes relationships among 919 natural kinds of primary igneous minerals, corresponding to 1665 mineral species approved by the International Mineralogical Association-minerals that are associated with the wide range of igneous rock types through 4.566 billion years of Earth history. A systematic survey of the mineral modes of 1850 varied igneous rocks from around the world reveals that 115 of these mineral kinds are frequent major and/or accessory phases. Of these most common primary igneous minerals, 69 are silicates, 19 are oxides, 13 are carbonates, and 6 are sulfides. Collectively, these 115 minerals incorporate at least 33 different essential chemical elements.

Subduction related to the ancient supercontinent cycle is poorly constrained by mantle samples. Sublithospheric diamond crystallization records the release of melts from subducting oceanic lithosphere at 300-700km depths1,2 and is especially suited to tracking the timing and effects of deep mantle processes on supercontinents. Here we show that four isotope systems (Rb-Sr, Sm-Nd, U-Pb and Re-Os) applied to Fe-sulfide and CaSiO3 inclusions within 13 sublithospheric diamonds from Juina (Brazil) and Kankan (Guinea) give broadly overlapping crystallization ages from around 450 to 650 million years ago. The intracratonic location of the diamond deposits on Gondwana and the ages, initial isotopic ratios, and trace element content of the inclusions indicate formation from a peri-Gondwanan subduction system. Preservation of these Neoproterozoic-Palaeozoic sublithospheric diamonds beneath Gondwana until its Cretaceous breakup, coupled with majorite geobarometry3,4, suggests that they accreted to and were retained in the lithospheric keel for more than 300Myr during supercontinent migration. We propose that this process of lithosphere growth-with diamonds attached to the supercontinent keel by the diapiric uprise of depleted buoyant material and pieces of slab crust-could have enhanced supercontinent stability.

Simple Summary: Although life on earth is quite diverse, some biological molecules are common across all life forms, both extant and extinct, and thus are thought to have been present as life emerged. Identifying how such compounds could have formed prior to life is therefore a critical step in understanding the origin of life and the potential for life elsewhere in the solar system and beyond. One class of biomolecule crucial to modern life are nucleic acids, which carry the genetic code and are integral to cellular replication and function. In modern biology, cellular machinery synthesizes these molecules; however, prior to life's beginning, it is possible that naturally occurring minerals played a role in the synthesis and polymerization of these molecules. Only a few minerals have been tested thus far; here, we investigate a variety of minerals for their ability to promote elongation of ribonucleic acid in water. In doing so, both the minerals and their environments of formation are tested for their potential to promote elongation. We show that several newly tested minerals can promote synthesis, suggesting that a broader set of environments may have been able to host chemical reactions relevant to the origin of life than previously assumed. The origin of life on earth requires the synthesis of protobiopolymers in realistic geologic environments along strictly abiotic pathways that rely on inorganic phases (such as minerals) instead of cellular machinery to promote condensation. One such class of polymer central to biochemistry is the polynucleotides, and oligomerization of activated ribonucleotides has been widely studied. Nonetheless, the range of laboratory conditions tested to date is limited and the impact of realistic early Earth conditions on condensation reactions remains unexplored. Here, we investigate the potential for a variety of minerals to enhance oligomerization using ribonucleotide monomers as one example to model condensation under plausible planetary conditions. The results show that several minerals differing in both structure and composition enhance oligomerization. Sulfide minerals yielded oligomers of comparable lengths to those formed in the presence of clays, with galena being the most effective, yielding oligonucleotides up to six bases long. Montmorillonite continues to excel beyond other clays. Chemical pretreatment of the clay was not required, though maximum oligomer lengths decreased from similar to 11 to 6 bases. These results demonstrate the diversity of mineral phases that can impact condensation reactions and highlight the need for greater consideration of environmental context when assessing prebiotic synthesis and the origin of life.

Nitrite, an intermediate product of the oxidation of ammonia to nitrate (nitrification), accumulates in upper oceans, forming the primary nitrite maximum (PNM). Nitrite concentrations in the PNM are relatively low in the western North Pacific subtropical gyre (wNPSG), where eddies are frequent and intense. To explain these low nitrite concentrations, we investigated nitrification in cyclonic eddies in the wNPSG. We detected relatively low half-saturation constants (i.e., high substrate affinities) for ammonia and nitrite oxidation at 150 to 200 meter water depth. Eddy-induced displacement of high-affinity nitrifiers and increased substrate supply enhanced ammonia and nitrite oxidation, depleting ambient substrate concentrations in the euphotic zone. Nitrite oxidation is more strongly enhanced by the cyclonic eddies than ammonia oxidation, reducing concentrations and accelerating the turnover of nitrite in the PNM. These findings demonstrate a spatial decoupling of the two steps of nitrification in response to mesoscale processes and provide insights into physical-ecological controls on the PNM.

During photosynthesis, electron transport reactions generate and shuttle reductant to allow CO2 reduction by the Calvin-Benson-Bassham cycle and the formation of biomass building block in the so-called linear electron flow (LEF). However, in nature, environmental parameters like light intensity or CO2 availability can vary and quickly change photosynthesis rates, creating an imbalance between photosynthetic energy production and metabolic needs. In addition to LEF, alternative photosynthetic electron flows are central to allow photosynthetic energy to match metabolic demand in response to environmental variations. Microalgae arguably harbour one of the most diverse set of alternative electron flows (AEFs), including cyclic (CEF), pseudocyclic (PCEF) and chloroplast-to-mitochondria (CMEF) electron flow. While CEF, PCEF and CMEF have large functional overlaps, they differ in the conditions they are active and in their role for photosynthetic energy balance. Here, I review the molecular mechanisms of CEF, PCEF and CMEF in microalgae. I further propose a quantitative framework to compare their key physiological roles and quantify how the photosynthetic energy is partitioned to maintain a balanced energetic status of the cell. Key differences in AEF within the green lineage and the potential of rewiring photosynthetic electrons to enhance plant robustness will be discussed.