Eukaryotic ribosome assembly is an intricate process that involves four ribosomal RNAs, 80 ribosomal proteins, and over 200 biogenesis factors that take part in numerous interdependent steps. This complexity creates a large genetic space in which pathogenic mutations can occur. “Dead-end” ribosome intermediates that result from biogenesis errors are rapidly degraded, affirming the existence of quality control pathway(s) that monitor ribosome assembly. However, the factors that differentiate between on -path and dead-end intermediates are unknown. We engineered a system to perturb ribosome assembly in human cells and discovered that faulty ribosomes are degraded via the ubiquitin proteasome system. We identified ZNF574 as a key component of a novel quality control pathway, which we term the Ribosome Assembly Surveillance Pathway (RASP). Loss of ZNF574 results in the accumulation of faulty biogenesis intermediates that interfere with global ribosome production, further emphasizing the role of RASP in protein homeostasis and cellular health.

The first JWST spectroscopy of the luminous galaxy GN-z11 simultaneously established its redshift at z = 10.6 and revealed a rest-ultraviolet spectrum dominated by signatures of highly ionized nitrogen, which has so far defied clear interpretation. We present a reappraisal of this spectrum in the context of both detailed nebular modeling and nearby metal-poor reference galaxies. The N iv] emission enables the first nebular density measurement in an apparently predominantly star-forming galaxy at z > 10, revealing evidence for extremely high densities n(e) greater than or similar to 10(5) cm(-3). With a suite of photoionization models, we establish that regardless of the ionization mechanism and accounting for depletion and this density enhancement, gas substantially enriched in nitrogen ([N/O] = +0.52 assuming the nebular emission is dominated by star formation) is required to reproduce the observed lines. We compare the GN-z11 spectrum to local UV databases and highlight a unique nearby galaxy, Mrk 996, where a high concentration of Wolf-Rayet stars and their CNO-processed ejecta produce a UV spectrum remarkably similar in some respects to that of GN-z11 and the Sunburst Arc. Collating this evidence in the context of Galactic stellar abundances, we suggest that the peculiar nitrogenic features prominent in GN-z11 may be a unique signature of intense and densely clustered star formation in the evolutionary chain of the present-day globular clusters, consistent with in situ early enrichment with nuclear-processed stellar ejecta on a massive scale. Combined with insight from local galaxies, these and future JWST data open a powerful new window into the physical conditions of star formation and chemical enrichment at the highest redshifts.

Nearly a decade ago, we began to see indications that reionization-era galaxies power hard radiation fields rarely seen at lower redshift. Most striking were detections of nebular C IV emission in what appeared to be typical low-mass galaxies, requiring an ample supply of 48 eV photons to triply ionize carbon. We have obtained deep JWST/NIRSpec R = 1000 spectroscopy of the two z > 6 C IV-emitting galaxies known prior to JWST. Here, we present a rest-UV to optical spectrum of one of these two systems, the multiply-imaged z = 6.1 lensed galaxy RXCJ2248-ID. NIRCam imaging reveals two compact (<22 pc) clumps separated by 220 pc, with one comprising a dense concentration of massive stars (>10 400 M-circle dot yr(-1) kpc(-2)) formed in a recent burst. We stack spectra of 3 images of the galaxy (J = 24.8-25.9), yielding a very deep spectrum providing a high-S/N template of strong emission line sources at z > 6. The spectrum reveals narrow high-ionization lines (He II, C IV, N IV]) with line ratios consistent with powering by massive stars. The rest-optical spectrum is dominated by very strong emission lines ([O III] EW = 2800 angstrom), albeit with weak emission from low-ionization transitions ([O III]/[O II] = 184). The electron density is found to be very high (6.4-31.0 x 10(4) cm(-3)) based on three UV transitions. The ionized gas is metal poor (12+log(O/H)=7.43(-0.09)(+0.17)), yet highly enriched in nitrogen (log(N/O)=-0.39(-0.10)(+0.11)). The spectrum appears broadly similar to that of GNz11 at z = 10.6, without showing the same AGN signatures. We suggest that the hard radiation field and rapid nitrogen enrichment may be a short-lived phase that many z > 6 galaxies go through as they undergo strong bursts of star formation. We comment on the potential link of such spectra to globular cluster formation.

We present a novel technique for mapping single-phase observations of Cepheids in any given band into their time-averaged values, using strong priors on the known interrelations of the multiwavelength widths of Cepheid period-luminosity (PL) relations, combined with the physical ordering of individual Cepheids within and across the instability strip, as a function of temperature (or radius). The method is empirically calibrated and tested using high-precision published multiwavelength observations of Cepheids in the LMC. The example, given herein, takes a single-epoch B-band PL relation and transforms those random-phase observations to within +/- 0.05-0.06 mag of their time-averaged values. For high-precision single-phase data points, this method can transform single-phase magnitudes into mean magnitudes (without additional observations), bringing the statistical error budget for the PL relation at that wavelength down to the systematic floor. This technique is of particular importance for use with space-based facilities (e.g., Hubble Space Telescope or JWST) where limits on the availability of telescope time preclude dense phase coverage, often resulting in only single-epoch observations being available.

We use SEDz*-a code designed to chart the star formation histories (SFHs) of 6 < z < 12 galaxies-to analyze the spectral energy distributions (SEDs) of 894 galaxies with deep JWST/NIRCam imaging by JADES in the GOODS-S field. We show how SEDz* matches observed SEDs using stellar-population templates, graphing the contribution of each epoch by epoch to confirm the robustness of the technique. Very good SED fits for most SFHs demonstrate the compatibility of the templates with stars in the first galaxies-as expected, because their light is primarily from main-sequence A stars, free of post-main-sequence complexity, and insensitive to heavy-element compositions. We confirm earlier results from Dressler et al. (1) There are four types of SFHs: SFH1, burst; SFH2, stochastic; SFH3, "contiguous" (three epochs), and SFH4, "continuous" (four to six epochs). (2) Starbursts-both single and multiple-are predominant (similar to 70%) in this critical period of cosmic history, although longer SFHs (0.5-1.0 Gyr) contribute one-third of the accumulated stellar mass. These 894 SFHs contribute 10(11.14), 10(11.09), 10(11.00), and 10(10.60)M(circle dot) for SFH1-4, respectively, adding up to similar to 4 x 10(11)M(circle dot) by z = 6 for this field. We suggest that the absence of rising SFHs could be explained as an intense dust-enshrouded phase of star formation lasting tens of Myr that preceded each of the SFHs we measure. We find no strong dependencies of SFH type with the large-scale environment; however, the discovery of a compact group of 30 galaxies, 11 of which had first star formation at z = 11-12, suggests that long SFHs could dominate in rare, dense environments.

The abundance of H in planetary building blocks is of fundamental importance for constraining the evolution of the terrestrial planets. It is commonly assumed that chondrites are the principal sources of Earth's H; however, recent studies have suggested that primitive achondrites and achondrites may retain a small complement of H. There are few constraints on the H budgets of primitive achondrites, which represent the transition from unmelted to melted planetesimals, but prior work suggests that bulk parent body H contents are several orders of magnitude lower than typical chondritic values. Therefore, to provide further constraints on H retention during the transition from unmelted to melted planetesimals, we have measured the H contents of olivine, orthopyroxene, clinopyroxene, and plagioclase from a suite of acapulcoite-lodranite clan meteorites. Acapulcoitelodranite clan meteorites represent the "prototypical" primitive achondrite parent body and have bulk major element compositions more akin to the Earth than previously studied primitive achondrites (e.g., the ureilites). We find that the H2O contents of olivine (-5-12 mu g/g H2O), orthopyroxene (-3-10 mu g/g H2O), and clinopyroxene (-5-8 mu g/g H2O) are broadly similar, while plagioclase (-2.5-5 mu g/g H2O) tends to be offset to lower values. Using a simple, single -stage batch -melting model, we calculate a preferred maximum acapulcoitelodranite parent body H2O content of 38 mu g/g, which is similar to other estimates for primitive achondritic and achondritic parent bodies. Furthermore, assuming chondrite-like precursor materials, our data are consistent with efficient loss of H prior to or during the onset of melting of early -formed planetesimals. This requires that Earth's H -budget was dominated by building blocks that underwent minimal thermal processing.