TOP assay data were used to identify PFAS contaminationsources based on visualizing a two-component PCA and similaritiesin chemical composition.Per- and polyfluoroalkylsubstances (PFASs) are a class of synthetic,organic chemicals that contaminate drinking water and natural ecosystems.PFAS source apportionment is challenging because there are many sources,and standard analytical methods quantify fewer than 100 of the thousandsof PFASs in commerce. The total oxidizable precursor (TOP) assay augmentsthe number of PFASs that can be quantified and is increasingly incorporatedinto routine site investigation. Here we examine the ability of theTOP assay to identify PFAS sources, including aqueous film-formingfoam (AFFF) impacted sites, municipal wastewater treatment plants(WWTPs), and municipal solid waste landfills in 145 samples from 46locations and three countries. The bootstrapped mean composition ofPFASs from each source was dominated by precursors, particularly insamples from WWTPs where precursors that form short-chain perfluoroalkylcarboxylates during the TOP assay were most common. Compared to whenTOP assay data were excluded, inclusion of TOP assay data in dimension-reducingalgorithms, such as principal component analysis (PCA), improved separationamong sources. We converted the PCA tool into a web application thatallows users to initiate PFAS source apportionment efforts on datafrom sites where contaminant sources are unknown.

We present two transit observations of the similar to 870 K, 1.7 R circle plus super-Earth TOI-836b with JWST NIRSpec/G395H, resulting in a 2.8-5.2 mu m transmission spectrum. Using two different reduction pipelines, we obtain a median transit depth precision of 34 ppm for Visit 1 and 36 ppm for Visit 2, leading to a combined precision of 25 ppm in spectroscopic channels 30 pixels wide (similar to 0.02 mu m). We find that the transmission spectrum from both visits is well fit by a zero-sloped line, by fitting zero-sloped and sloped lines as well as step functions to our data. Combining both visits, we are able to rule out atmospheres with metallicities <250 times solar for an opaque pressure level of 0.1 bar, corresponding to mean molecular weights of less than or similar to 6 g mol-1. We therefore conclude that TOI-836b does not have an H2-dominated atmosphere, in possible contrast with its larger, exterior sibling planet, TOI-836 c. We recommend that future proposals to observe small planets exercise caution when requiring specific numbers of transits to rule out physical scenarios, particularly for high metallicities and planets around bright host stars, as PandExo predictions appear to be more optimistic than what the gains from additional transits implied by our data suggest.

Climate change is increasing the frequency and severity of short-term (similar to 1 y) drought events-the most common duration of drought-globally. Yet the impact of this intensification of drought on ecosystem functioning remains poorly resolved. This is due in part to the widely disparate approaches ecologists have employed to study drought, variation in the severity and duration of drought studied, and differences among ecosystems in vegetation, edaphic and climatic attributes that can mediate drought impacts. To overcome these problems and better identify the factors that modulate drought responses, we used a coordinated distributed experiment to quantify the impact of short-term drought on grassland and shrubland ecosystems. With a standardized approach, we imposed similar to a single year of drought at 100 sites on six continents. Here we show that loss of a foundational ecosystem function-aboveground net primary production (ANPP)-was 60% greater at sites that experienced statistically extreme drought (1-in-100-y event) vs. those sites where drought was nominal (historically more common) in magnitude (35% vs. 21%, respectively). This reduction in a key carbon cycle process with a single year of extreme drought greatly exceeds previously reported losses for grasslands and shrublands. Our global experiment also revealed high variability in drought response but that relative reductions in ANPP were greater in drier ecosystems and those with fewer plant species. Overall, our results demonstrate with unprecedented rigor that the global impacts of projected increases in drought severity have been significantly underestimated and that drier and less diverse sites are likely to be most vulnerable to extreme drought.

Irrigation is a land management practice with major environmental impacts. However, global energy consumption and carbon emissions resulting from irrigation remain unknown. We assess the worldwide energy consumption and carbon emissions associated with irrigation,while also measuring the potential energy and carbon reductions achievable through the adoption of efficient and low-carbon irrigation practices. Currently, irrigation contributes 216 million metric tons of CO2 emissions and consumes 1896 petajoules of energy annually, representing 15% of greenhouse gas emissions and energy utilized in agricultural operations. Despite only 40% of irrigated agriculture relies on groundwatersources, groundwater pumping accounts for 89% of the total energy consumption in irrigation. Projections indicate that future expansion of irrigation could lead to a 28% increase in energy usage. Embracing highly efficient, low-carbon irrigation methods has the potential to cut energy consumption in half and reduce CO2 emissions by 90%. However, considering country-specific feasibility of mitigation options, global CO2 emissions may only see a 55% reduction. Our research offers comprehensive insights into the energy consumption and carbon emissions associated with irrigation, contributing valuable information that can guide assessments of the viability of irrigation in enhancing adaptive capacity within the agricultural sector.

The potential of enhanced agricultural management practices to drive sustainability is rarely quantified at grassroots level. Here we analyse nitrogen use and loss in Chinese cropland, drawing from data collected in 2,238,550 sites in two national agricultural pollution source censuses from 2007 to 2017. We find an upswing of 10% in crop yields and an 8% reduction in nitrogen pollution during this period, owing to the promotion and adoption of various management practices (including the combination of organic and chemical fertilizers, straw recycling and deep placement of fertilizer). These practices have collectively contributed to an 18% increase in nitrogen use efficiency in the country. By fully embracing them, we project that annual cropland pollution could be further reduced by up to 1.4 Mt of nitrogen without compromising crop yields. Environmental and human health benefits are projected to consistently outweigh implementation costs in the future, with total benefits reaching US$15 billion.

Background: Temperate subalpine lakes recovering from eutrophication in central Europe are experiencing harmful blooms due to the proliferation of Planktothrix rubescens, a potentially toxic cyanobacteria. To optimize the management of cyanobacteria blooms there is the need to better comprehend the combination of factors influencing the diversity and dominance of cyanobacteria and their impact on the lake's ecology. The goal of this study was to characterize the diversity and seasonal dynamics of cyanobacteria communities found in a water column of Lake Geneva, as well as the associated changes on bacterioplankton abundance and composition.Methods: We used 16S rRNA amplicon high throughput sequencing on more than 200 water samples collected from surface to 100 meters deep monthly over 18 months. Bacterioplankton abundance was determined by quantitative PCR and PICRUSt predictions were used to explore the functional pathways present in the community and to calculate functional diversity indices.Results: The obtained results confirmed that the most dominant cyanobacteria in Lake Geneva during autumn and winter was Planktothrix (corresponding to P. rubescens). Our data also showed an unexpectedly high relative abundance of picocyanobacterial genus Cyanobium, particularly during summertime. Multidimensional scaling of Bray Curtis dissimilarity revealed that the dominance of P. rubescens was coincident with a shift in the bacterioplankton community composition and a significant decline in bacterioplankton abundance, as well as a temporary reduction in the taxonomic and PICRUSt2 predicted functional diversity.Conclusion: Overall, this study expands our fundamental understanding of the seasonal dynamics of cyanobacteria communities along a vertical column in Lake Geneva and the ecology of P. rubescens, ultimately contributing to improve our preparedness against the potential occurrence of toxic blooms in the largest lake of western Europe.

While natural communities can contain hundreds of species, modern coexistence theory focuses primarily on species pairs. Alternatively, the structural stability approach considers the feasibility of equilibria, gaining scalability to larger communities but sacrificing information about dynamic stability. Three-species competitive communities are a bridge to more-diverse communities. They display novel phenomena while remaining amenable to mathematical analysis, but remain incompletely understood. Here, we combine these approaches to identify the key quantities that determine three-species competition outcomes. We show that pairwise niche overlap and fitness differences are insufficient to completely characterize competitive outcomes, which requires a strictly triplet-wise quantity: cyclic asymmetry, which underlies intransitivity. Low pairwise niche overlap stabilizes the triplet, while high fitness differences promote competitive exclusion. The effect of cyclic asymmetry on stability is complex and depends on pairwise niche overlap. In summary, we elucidate how pairwise niche overlap, fitness differences and cyclic asymmetry determine three-species competition outcomes.

We present optical observations of the Swift short-duration gamma-ray burst (GRB) GRB 161104A and its host galaxy at z = 0.793 +/- 0.003. We model the multiband photometry and spectroscopy with the stellar population inference code Prospector and explore the posterior using nested sampling. We find a mass-weighted age of t(m) = 2.12(-0.21)(+0.23) Gyr, stellar mass of log(M/M-circle dot) = 10.21 +/- 0.04, metallicity of log(Z/Z(circle dot)) = 0.08(-0.06)(+0.05), dust extinction of A(V) = 0.08(-0.05)(+0.08), and low star formation rate of 9.9 x 10(-2) Me yr(-1). These properties, along with a prominent 4000 angstrom break and optical absorption lines, classify this host as an early-type, quiescent galaxy. Using Dark Energy Survey galaxy catalogs, we demonstrate that the host of GRB 161104A resides on the outskirts of a galaxy cluster at z approximate to 0.8, situated approximate to 1 Mpc from the likely brightest cluster galaxy. We also present new modeling for 20 additional short GRB hosts (approximate to 33% of which are early-type galaxies), finding population medians of log(M/M-circle dot) = 9.94(-0.98)(+0.88) and t(m) = 1.07(-0.67)(+1.98) Gyr (68% confidence). We further find that the host of GRB 161104A is more distant, less massive, and younger than the four other short GRB hosts known to be associated with galaxy clusters. Cluster short GRBs have faint afterglows, in the lower approximate to 11% (approximate to 30%) of observed X-ray (optical) luminosities. We place a lower limit on the fraction of short GRBs in galaxy clusters versus those in the field of approximate to 5%-13%, consistent with the fraction of stellar mass of approximate to 10%-20% in galaxy clusters at redshifts 0.1 <= z <= 0.8. Future studies that take advantage of wider-field and deeper cluster surveys are needed to understand the true rate of short GRBs in clusters and their effect on heavy-element enrichment in the intracluster medium.

The bioluminescent bacterium Vibrio fischeri forms a mutually beneficial symbiosis with the Hawaiian bobtail squid, Euprymna scolopes, in which the bacteria, housed inside a specialized light organ, produce light used by the squid in its nocturnal activities. Upon hatching, E. scolopes juveniles acquire V. fischeri from the seawater through a complex process that requires, among other factors, chemotaxis by the bacteria along a gradient of N-acetylated sugars into the crypts of the light organ, the niche in which the bacteria reside. Once inside the light organ, V. fischeri transitions into a symbiotic, sessile state in which the quorum-signaling regulator LitR induces luminescence. In this work we show that expression of litR and luminescence are repressed by a homolog of the V. cholerae virulence factor TcpP, which we have named HbtR. Further, we demonstrate that LitR represses genes involved in motility and chemotaxis into the light organ and activates genes required for exopolysaccharide production. ImportanceTcpP homologs are widespread throughout the Vibrio genus; however, the only protein in this family described thus far is a V. cholerae virulence regulator. Here we show that HbtR, the TcpP homolog in V. fischeri, has both a biological role and regulatory pathway completely unlike that in V. cholerae. Through its repression of the quorum-signaling regulator LitR, HbtR affects the expression of genes important for colonization of the E. scolopes light organ. While LitR becomes activated within the crypts, and upregulates luminescence and exopolysaccharide genes and downregulates chemotaxis and motility genes, it appears that HbtR, upon expulsion of V. fischeri cells into seawater, reverses this process to aid the switch from a symbiotic to a planktonic state. The possible importance of HbtR to the survival of V. fischeri outside of its animal host may have broader implications for the ways in which bacteria transition between often vastly different environmental niches.