Recent studies show that pre-follicular mouse oogenesis takes place in germline cysts, highly conserved groups of oogonial cells connected by intercellular bridges that develop as nurse cells as well as an oocyte. Long studied in Drosophila and insect gametogenesis, female germline cysts acquire cytoskeletal polarity and traffic centrosomes and organelles between nurse cells and the oocyte to form the Balbiani body, a conserved marker of polarity. Mouse oocyte development and nurse cell dumping are supported by dynamic, cell-specific programs of germline gene expression. High levels of perinatal germ cell death in this species primarily result from programmed nurse cell turnover after transfer rather than defective oocyte production. The striking evolutionary conservation of early oogenesis mechanisms between distant animal groups strongly suggests that gametogenesis and early embryonic development in vertebrates and invertebrates share even more in common than currently believed.

Presolar grains are trace components in chondrite matrices. Their abundances and compositions have been systematically studied in carbonaceous chondrites but rarely in situ in other major chondrite classes. We have conducted a NanoSIMS isotopic search for presolar grains with O-and C-anomalous iso-topic compositions in the matrices of the unequilibrated ordinary chondrites Semarkona (LL3.00), Meteorite Hills 00526 (L/LL3.05), and Northwest Africa 8276 (L3.00). The matrices of even the most primitive ordinary chondrites have been aqueously altered and/or thermally metamorphosed, destroying their presolar grain populations to varying extents. In addition to randomly placed isotope maps, we specifically targeted recently reported, relatively pristine Semarkona matrix areas to better explore the original inventory of presolar grains in this meteorite. In all samples, we found a total of 122 O-anomalous grains (silicates + oxides), 79 SiC grains, and 22 C-anomalous carbonaceous grains (organics, graphites). Average matrix-normalized abundances with 1 sigma uncertainties are 151(-46)( +50 )ppm O-anomalous grains, 53(-12)(+14) ppm SiC grains and 56(-14)(+19 )ppm carbonaceous grains in Semarkona, 55(-10)( +11) ppm (O-anom.), 22(-4)(+5 ) ppm (SiC) and 3(-1)(+2) ppm (carb.) in MET 00526 and 12(-3)(+6) ppm (O-anom.), 15(-5)(+7 )ppm (SiC) and 1 thorn 3 ?1 ppm (carb.) in NWA 8276. In relatively pristine ordinary chondrites and in primitive carbonaceous and C-ungrouped chondrites, the O and C isotopic composition of presolar grains and their matrix-normalized abundances are similar, despite the likely differences in chondrite-formation time and nebular location. These results suggest a relatively homogenous distribution of presolar dust across major chondrite-forming reservoirs in the solar nebula. Secondary asteroidal processes are mainly responsible for differences in presolar grain abundances between and within chondrites, highlighting the need to identify and target the most pristine chondrite matrices for such studies. (C) 2022 Elsevier Ltd. All rights reserved.

Protein coordinated iron-sulfur clusters drive electron flow within metabolic pathways for organisms throughout the tree of life. It is not known how iron-sulfur clusters were first incorporated into proteins. Structural analogies to iron-sulfide minerals present on early Earth, suggest a connection in the evolution of both proteins and minerals. The availability of large protein and mineral crystallographic structure data sets, provides an opportunity to explore co-evolution of proteins and minerals on a large-scale using informatics approaches. However, quantitative comparisons are confounded by the infinite, repeating nature of the mineral lattice, in contrast to metal clusters in proteins, which are finite in size. We address this problem using the Niggli reduction to transform a mineral lattice to a finite, unique structure that when translated reproduces the crystal lattice. Protein and reduced mineral structures were represented as quotient graphs with the edges and nodes corresponding to bonds and atoms, respectively. We developed a graph theory-based method to calculate the maximum common connected edge subgraph (MCCES) between mineral and protein quotient graphs. MCCES can accommodate differences in structural volumes and easily allows additional chemical criteria to be considered when calculating similarity. To account for graph size differences, we use the Tversky similarity index. Using consistent criteria, we found little similarity between putative ancient iron-sulfur protein clusters and iron-sulfur mineral lattices, suggesting these metal sites are not as evolutionarily connected as once thought. We discuss possible evolutionary implications of these findings in addition to suggesting an alternative proxy, mineral surfaces, for better understanding the coevolution of the geosphere and biosphere.

Although lacking an adaptive immune system and often living in habitats with dense and diverse bacterial populations, marine invertebrates thrive in the presence of potentially challenging microbial pathogens. However, the mechanisms underlying this resistance remain largely unexplored and promise to reveal novel strategies of microbial resistance. Here, we provide evidence that a mud-dwelling clam, Meretrix petechialis, synthesizes, stores, and secretes the antibiotic erythromycin. Liquid chromatography coupled with mass spectrometry, immunocytochemistry, fluorescence in situ hybridization, RNA interference, and enzyme-linked immunosorbent assay revealed that this potent macrolide antimicrobial, thought to be synthesized only by microorganisms, is produced by specific mucus-rich cells beneath the clam's mantle epithelium, which interfaces directly with the bacteria-rich environment. The antibacterial activity was confirmed by bacteriostatic assay. Genetic, ontogenetic, phylogenetic and genomic evidence, including genotypic segregation ratios in a family of full siblings, gene expression in clam larvae, phylogenetic tree, and synteny conservation in the related genome region further revealed that the genes responsible for erythromycin production are of animal origin. The detection of this antibiotic in another clam species showed that the production of this macrolide is not exclusive to M. petechialis and may be a common strategy among marine invertebrates. The finding of erythromycin production by a marine invertebrate offers a striking example of convergent evolution in secondary metabolite synthesis between the animal and bacterial domains. These findings open the possibility of engineering-animal tissues for the localized production of an antibacterial secondary metabolite.

The Glen Torridon (GT) region in Gale crater, Mars is a region with strong clay mineral signatures inferred from orbital spectroscopy. The CheMin X-ray diffraction (XRD) instrument onboard the Mars Science Laboratory rover, Curiosity, measured some of the highest clay mineral abundances to date within GT, complementing the orbital detections. GT may also be unique because in the XRD patterns of some samples, CheMin identified new phases, including: (a) Fe-carbonates, and (b) a phase with a novel peak at 9.2 angstrom. Fe-carbonates have been previously suggested from other instruments onboard, but this is the first definitive reporting by CheMin of Fe-carbonate. This new phase with a 9.2 angstrom reflection has never been observed in Gale crater and may be a new mineral for Mars, but discrete identification still remains enigmatic because no single phase on Earth is able to account for all of the GT mineralogical, geochemical, and sedimentological constraints. Here, we modeled XRD profiles and propose an interstratified clay mineral, specifically greenalite-minnesotaite, as a reasonable candidate. The coexistence of Fe-carbonate and Fe-rich clay minerals in the GT samples supports a conceptual model of a lacustrine groundwater mixing environment. Groundwater interaction with percolating lake waters in the sediments is common in terrestrial lacustrine settings, and the diffusion of two distinct water bodies within the subsurface can create a geochemical gradient and unique mineral front in the sediments. Ultimately, the proximity to this mixing zone may have controlled the secondary minerals preserved in sedimentary rocks exposed in GT.

The Dark Energy Camera is a new imager with a 2 degrees.2 diameter field of view mounted at the prime focus of the Victor M. Blanco 4 m telescope on Cerro Tololo near La Serena, Chile. The camera was designed and constructed by the Dark Energy Survey Collaboration. and meets or exceeds the stringent requirements designed for the wide-field and supernova surveys for which the collaboration uses it. The camera consists of a five-element optical corrector, seven filters, a shutter with a 60 cm aperture, and a charge-coupled device (CCD) focal plane of 250 mu m thick fully depleted CCDs cooled inside a vacuum Dewar. The 570 megapixel focal plane comprises 62 2k x 4k CCDs for imaging and 12 2k x 2k CCDs for guiding and focus. The CCDs have 15 mu m x 15 mu m pixels with a plate scale of 0 ''.263 pixel(-1). A hexapod system provides state-of-the-art focus and alignment capability. The camera is read out in 20 s with 6-9 electron. readout noise. This paper provides a technical description of the camera's engineering, construction, installation, and current status.

The clinical and largely unpredictable heterogeneity of phenotypes in patients with mitochondrial disorders demonstrates the ongoing challenges in the understanding of this semi-autonomous organelle in biology and disease. Previously, we used the gene-breaking transposon to create 1200 transgenic zebrafish strains tagging protein-coding genes (Ichino et al., 2020), including the lrpprc locus. Here, we present and characterize a new genetic revertible animal model that recapitulates components of Leigh Syndrome French Canadian Type (LSFC), a mitochondrial disorder that includes diagnostic liver dysfunction. LSFC is caused by allelic variations in the LRPPRC gene, involved in mitochondrial mRNA polyadenylation and translation. lrpprc zebrafish homozygous mutants displayed biochemical and mitochondrial phenotypes similar to clinical manifestations observed in patients, including dysfunction in lipid homeostasis. We were able to rescue these phenotypes in the disease model using a liver-specific genetic model therapy, functionally demonstrating a previously under-recognized critical role for the liver in the pathophysiology of this disease.

Stomata, cellular valves found on the surfaces of aerial plant tissues, present a paradigm for studying cell fate and patterning in plants. A highly conserved core set of related basic helix-loop-helix (bHLH) transcription factors regulate stomatal development across diverse species. We characterized BdFAMA in the temperate grass Brachypodium distachyon and found this late-acting transcription factor was necessary and sufficient for specifying stomatal guard cell fate, and unexpectedly could also induce the recruitment of subsidiary cells in the absence of its paralogue, BdMUTE. The overlap in function is paralleled by an overlap in expression pattern and by unique regulatory relationships between BdMUTE and BdFAMA. To better appreciate the relationships among the Brachypodium stomatal bHLHs, we used in vivo proteomics in developing leaves and found evidence for multiple shared interaction partners. We reexamined the roles of these genes in Arabidopsis thaliana by testing genetic sufficiency within and across species, and found that while BdFAMA and AtFAMA can rescue stomatal production in Arabidopsis fama and mute mutants, only AtFAMA can specify Brassica -specific myrosin idioblasts. Taken together, our findings refine the current models of stomatal bHLH function and regulatory feedbacks amongst paralogues within grasses as well as across the monocot/dicot divide.