Sheet 1: Relative expression values of ssrA from bacteria cells fraction or OMV fractions. Cells grown in three different media: a tryptone-based medium (LBS) or LBS with the addition of either glycerol (32.6 mM) or GlcNAc (10 mM). S1A Fig. Sheet 2: OD600 values over 24 h of bacteria growth in tryptone-based medium (LBS). S1A Fig. Sheet 3: OD600 values over 24 h of bacteria growth in minimum medium. S1A Fig. Sheet 4: Motility in soft agar of WT or DeltassrA cells measured as the diameter of the outer migration ring at 3 and 7 h post inoculation. S2B Fig. Sheet 5: Respiration rates of WT, DeltassrA, DeltassrA + ssrA, and a nonluminescent lux-deletion mutant (Deltalux) normalized to OD600. Fig 2C. Sheet 6: RCI between WT and DeltassrA in co-inoculated light organs after 24, 48, and 72 h. S2D Fig. Sheet 7: Relative expression values of ssrA and smpB. S2E Fig. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OD600, optical density at 600 nm; OMV, outer membrane vesicle; RCI, relative competitive index; WT, wild type. (XLSX) Copyright: CC BY 4.0

Sheet 1: Survival proportions of juvenile squid colonized by either WT, DeltassrA, DeltassrA + ssrA, or DeltasmpB. Fig 4B, S6B Fig. Sheet 2: Dry weight of juvenile squid immediately after hatching ("Hatch") or at 4 d post hatching when kept APO or colonized with WT, DeltassrA, or a nonluminescent mutant (Deltalux) strain. Fig 4C. Sheet 3: Quantification of internal yolk-sac area of juvenile squid immediately after hatching ("Hatch") or at 2 d post hatching colonized with WT or DeltassrA. Fig 4D. APO, aposymbiotic; WT, wild type. (XLSX) Copyright: CC BY 4.0

Sheet 1: Counts in OMV and hemolymph samples. Sheet 2: Numerical values for Fig 1B. Sheet 3: Differential-expression analysis (Fig 1C). OMV, outer membrane vesicle; RNA-seq, RNA sequencing. (XLSX) Copyright: CC BY 4.0

. Copyright: CC BY 4.0

Sheet 1: Relative expression values of C3. Fig 5B. Sheet 1: Relative expression values of RIG-I. Fig 5B. C3, complement protein 3; RIG-I, retinoic-acid inducible gene-I. (XLSX) Copyright: CC BY 4.0

Sheet 1: Survival proportion of juvenile squid that were either single-colonized by WT or DeltassrA, or co-colonized at a 1:1 inoculum ratio with both WT and DeltassrA (n = 60). S6C Fig. Sheet 2: Respiration rates of newly hatched squid ("Hatch"), or of animals after 24 h, that were either maintained APO or colonized by WT, DeltassrA, or Deltalux strains. S6D Fig. Sheet 3: Internal yolk-sac area values, 2 d post colonization with WT, DeltassrA, its complement (DeltassrA + ssrA), the nonluminescent mutant (Deltalux), or DeltasmpB strains. S6E Fig. Sheet 4: Quantification of laccase-3 signal by HCR using relative fluorescence intensity of a Z-series image of the light organ. S7A Fig. Sheet 5: Quantification of laccase-3 presence by HCR fluorescence signal intensity from a Z-series image of light organs, 3 h after incubation with WT or DeltassrA OMVs. S7B Fig. APO, aposymbiotic; HCR, hybridization chain reaction; OMV, outer membrane vesicle; WT, wild type. (XLSX) Copyright: CC BY 4.0

Sheet 1: CFU per squid. Fig 2A. Sheet 2: Number of hemocytes trafficking into the light-organ appendages after 16 and 18 h post colonization. Fig 2B. Sheet 3: Number of hemocytes trafficking into the light-organ appendages after 3 h inoculation with WT or DeltassrA OMVs. Fig 2C. Sheet 4: Number of apoptotic nuclei per appendage. Fig 2D. Sheet 5: RLU per CFU of symbionts either within the light organ, or within a homogenate of the light organ, of a 24-h juvenile. Fig 2E. CFU, colony-forming units; OMV, outer membrane vesicle; RLU, relative light units. (XLSX) Copyright: CC BY 4.0

In this Review, Visick, Stabb and Ruby describe recent advances in understanding the squid-vibrio symbiosis from the symbiont's perspective.