Background MacArthur and Wilson's theory of island biogeography has been a foundation for obtaining testable predictions from models of community assembly and for developing models that integrate across scales and disciplines. Historically, however, these developments have focused on integration across ecological and macroevolutionary scales and on predicting patterns of species richness, abundance distributions, trait data and/or phylogenies. The distribution of genetic variation across species within a community is an emerging pattern that contains signatures of past population histories, which might provide an historical lens for the study of contemporary communities. As intraspecific genetic diversity data become increasingly available at the scale of entire communities, there is an opportunity to integrate microevolutionary processes into our models, moving towards development of a genetic theory of island biogeography. Motivation/goal We aim to promote the development of process-based biodiversity models that predict community genetic diversity patterns together with other community-scale patterns. To this end, we review models of ecological, microevolutionary and macroevolutionary processes that are best suited to the creation of unified models, and the patterns that these predict. We then discuss ongoing and potential future efforts to unify models operating at different organizational levels, with the goal of predicting multidimensional community-scale data including a genetic component. Main conclusions Our review of the literature shows that despite recent efforts, further methodological developments are needed, not only to incorporate the genetic component into existing island biogeography models, but also to unify processes across scales of biological organization. To catalyse these developments, we outline two potential ways forward, adopting either a top-down or a bottom-up approach. Finally, we highlight key ecological and evolutionary questions that might be addressed by unified models including a genetic component and establish hypotheses about how processes across scales might impact patterns of community genetic diversity.

Seed development is important for agriculture productivity. We demonstrate that brassinosteroid (BR) plays crucial roles in determining the size, mass, and shape of Arabidopsis (Arabidopsis thaliana) seeds. The seeds of the BR-deficient mutant de-etiolated2 (det2) are smaller and less elongated than those of wild-type plants due to a decreased seed cavity, reduced endosperm volume, and integument cell length. The det2 mutant also showed delay in embryo development, with reduction in both the size and number of embryo cells. Pollination of det2 flowers with wild-type pollen yielded seeds of normal size but still shortened shape, indicating that the BR produced by the zygotic embryo and endosperm is sufficient for increasing seed volume but not for seed elongation, which apparently requires BR produced from maternal tissues. BR activates expression of SHORT HYPOCOTYL UNDER BLUE1, MINISEED3, and HAIKU2, which are known positive regulators of seed size, but represses APETALA2 and AUXIN RESPONSE FACTOR2, which are negative regulators of seed size. These genes are bound in vivo by the BR-activated transcription factor BRASSINAZOLE-RESISTANT1 (BZR1), and they are known to influence specific processes of integument, endosperm, and embryo development. Our results demonstrate that BR regulates seed size and seed shape by transcriptionally modulating specific seed developmental pathways.

Quasars represent a brief phase in the life cycle of most massive galaxies, but the evolutionary connection between central black holes and their host galaxies remains unclear. While quasars are active and shining brighter than the Compton-limit luminosity, their radiation heats the surrounding medium to the Compton temperature, forming Compton spheres extending to the Stromgren radius of Fe26+/He2+. After the quasars shut off, their "afterglow" can be detected through three signatures: (1) an extended X-ray envelope, with a characteristic temperature of similar to 3 x 10(7) (2) Ly alpha and Ly beta lines and the K edge of Fe26+, and (3) nebulosity from hydrogen and helium recombination emission lines. We discuss the possibility of detecting these signatures using Chandra, the planned X-Ray Evolving Universe Spectroscopy mission, and ground-based optical telescopes. The luminosity and size of quasar afterglows can be used to constrain the lifetime of quasars.

In the past decade, the group V-VI compounds have been widely investigated due to their excellent properties and applications. It is now accepted that diverse stoichiometry can yield new compounds with unanticipated properties, uncovering potentially new physicochemical mechanisms. However, in this group, aside from the conventional A(2)B(3)-type, no other energetically stable stoichiometry has been reported yet. Here, we report that Bi2S3 is unstable and decomposes into stoichiometric BiS2 and BiS with different Bi valence states upon compression. Encouragingly, we successfully synthesized the predicted BiS2 phase and thus, confirmed its existence. Our current calculations reveal that the found BiS2 phase is a semimetal, associated with the increased concentration of nonmetallic S. The present results represent the first counterintuitive stable stoichiometry of group V-VI and provide a good example in designing and synthesizing new compounds under compression.

We report initial results from a large Gemini program to observe z greater than or similar to 5.7 quasars with GNIRS near-IR spectroscopy. Our sample includes 50 quasars with simultaneous similar to 0.85-2.5 mu m spectra covering the rest-frame ultraviolet and major broad emission lines from Ly alpha to Mg II. We present spectral measurements for these quasars and compare with their lower redshift counterparts at z = 1.5-2.3. We find that when quasar luminosity is matched, there are no significant differences between the rest-UV spectra of z greater than or similar to 5.7 quasars and the low-z comparison sample. High-z quasars have similar continuum and emission line properties and occupy the same region in the black hole mass and luminosity space as the comparison sample, accreting at an average Eddington ratio of similar to 0.3. There is no evidence for super-Eddington accretion or hypermassive (> 10(10) M-circle dot) black holes within our sample. We find a mild excess of quasars with weak C IV lines relative to the control sample. Our results, corroborating earlier studies but with better statistics, demonstrate that these high-z quasars are already mature systems of accreting supermassive black holes operating with the same physical mechanisms as those at lower redshifts.

This is the third paper in a series aimed at finding reionization-era quasars with the combination of DESI Legacy imaging Surveys (DELS), the Pan-STARRS1 (PS1) Survey, and near-infrared imaging surveys, such as the UKIRT Hemisphere Survey (UHS), as well as the Wide-field Infrared Survey Explorer (WISE) mid-infrared survey. In this paper, we describe the updated quasar candidate selection procedure, report the discovery of 16 quasars at 6.4 less than or similar to z less than or similar to 6.9 from an area of similar to 13,020 deg(2), and present the quasar luminosity function (QLF) at z similar to 6.7. The measured QLF follows Phi(L-1450) proportional to L-1450(-2.35) in the magnitude range 27.6 < M-1450 < 25.5. We determine the quasar comoving spatial density at < z > - 6.7 and M-1450 < -26.0 to be 0.39 +/- 0.11 Gpc(-3) and find the exponential density evolution parameter to be k = -0.78 +/- 0.18 from z similar to 6 to z similar to 6.7, corresponding to a rapid decline by a factor of similar to 6 per unit redshift toward earlier epochs. This indicates that the rapid decline of quasar spatial density at z > 5 that was found by previous works continues to z > 6, at a rate significantly faster than the average decline rate between z similar to 3 and 5. We measured quasar comoving emissivity at z similar to 6.7, which indicates that high-redshift quasars are highly unlikely to make a significant contribution to hydrogen reionization. The broad absorption line quasar fraction at z greater than or similar to 6.5 is measured to be greater than or similar to 22%. In addition, we also report the discovery of six additional quasars at z similar to 6 in the Appendix.

Acylsugars are a class of plant defense compounds produced across many distantly related families. Members of the horticulturally important morning glory (Convolvulaceae) family produce a diverse sub-class of acylsugars called resin glycosides (RGs), which comprise oligosaccharide cores, hydroxyacyl chain(s), and decorating aliphatic and aromatic acyl chains. While many RG structures are characterized, the extent of structural diversity of this class in different genera and species is not known. In this study, we asked whether there has been lineage-specific diversification of RG structures in different Convolvulaceae species that may suggest diversification of the underlying biosynthetic pathways. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was performed from root and leaf extracts of 26 species sampled in a phylogeny-guided manner. LC-MS/MS revealed thousands of peaks with signature RG fragmentation patterns with one species producing over 300 signals, mirroring the diversity in Solanaceae-type acylsugars. A novel RG from Dichondra argentea was characterized using Nuclear Magnetic Resonance spectroscopy, supporting previous observations of RGs with open hydroxyacyl chains instead of closed macrolactone ring structures. Substantial lineage-specific differentiation in utilization of sugars, hydroxyacyl chains, and decorating acyl chains was discovered, especially among Ipomoea and Convolvulus - the two largest genera in Convolvulaceae. Adopting a computational, knowledge-based strategy, we further developed a high-recall workflow that successfully explained ~72% of the MS/MS fragments, predicted the structural components of 11/13 previously characterized RGs, and partially annotated ~45% of the RGs. Overall, this study improves our understanding of phytochemical diversity and lays a foundation for characterizing the evolutionary mechanisms underlying RG diversification.

α- and β-tubulin form heterodimers, with GTPase activity, that assemble into microtubules. Like other GTPases, the nucleotide-bound state of tubulin heterodimers controls whether the molecules are in a biologically active or inactive state. While -tubulin in the heterodimer is constitutively bound to GTP, β-tubulin can be bound to either GDP (GDP-tubulin) or GTP (GTP-tubulin). GTP-tubulin hydrolyzes its GTP to GDP following assembly into a microtubule and, upon disassembly, must exchange its bound GDP for GTP to participate in subsequent microtubule polymerization. Tubulin dimers have been shown to exhibit rapid intrinsic nucleotide exchange in vitro, leading to a commonly accepted belief that a tubulin guanine nucleotide exchange factor (GEF) may be unnecessary in cells. Here, we use quantitative binding assays to show that BuGZ, a spindle assembly factor, binds tightly to GDP-tubulin, less tightly to GTP-tubulin, and weakly to microtubules. We further show that BuGZ promotes the incorporation of GTP into tubulin using a nucleotide exchange assay. The discovery of a tubulin GEF suggests a mechanism that may aid rapid microtubule assembly dynamics in cells.

How, when, and why organisms age are fascinating issues that can only be fully addressed by adopting an evolutionary perspective. Consistently, the main evolutionary theories of ageing, namely the Mutation Accumulation theory, the Antagonistic Pleiotropy theory, and the Disposable Soma theory, have formulated stimulating hypotheses that structure current debates on both the proximal and ultimate causes of organismal ageing. However, all these theories leave a common area of biology relatively under-explored. The Mutation Accumulation theory and the Antagonistic Pleiotropy theory were developed under the traditional framework of population genetics, and therefore are logically centred on the ageing of individuals within a population. The Disposable Soma theory, based on principles of optimising physiology, mainly explains ageing within a species. Consequently, current leading evolutionary theories of ageing do not explicitly model the countless interspecific and ecological interactions, such as symbioses and host-microbiomes associations, increasingly recognized to shape organismal evolution across the Web of Life. Moreover, the development of network modelling supporting a deeper understanding on the molecular interactions associated with ageing within and between organisms is also bringing forward new questions regarding how and why molecular pathways associated with ageing evolved. Here, we take an evolutionary perspective to examine the effects of organismal interactions on ageing across different levels of biological organisation, and consider the impact of surrounding and nested systems on organismal ageing. We also apply this perspective to suggest open issues with potential to expand the standard evolutionary theories of ageing.