Variations in the Dolivo-Dobrovol'sky symmetry index for minerals through time reveal several factors that influence the emergence of crystalline symmetry in natural processes. Of special interest in this regard are the numerous paragenetic modes-different processes of mineral genesis that reflect changes in physical, chemical, and ultimately biological environments that foster the emergence of new mineral species. Here, we consider the roles of hydrogen content, rarity, formation temperature and pressure, and age on the average symmetry of minerals from 57 different modes of formation (i.e., paragenetic modes). We find four significant trends in the average mineral symmetry index for all minerals in each paragenetic mode: specifically, this average index is (1) lower for minerals with greater hydrogen content; (2) greater for minerals formed at higher pressure; (3) lower for minerals of greater rarity; and (4) greater for older paragenetic modes. These findings elucidate some of the intricate relationships among paragenetic modes, average mineral attributes, and the Dolivo-Dobrovol'sky symmetry index, providing insights into the geological processes governing mineral formation.

The solidification of a deep magma ocean occurred early in Earth's history. Although the initial amount of H2O in Earth's magma ocean is predicted to be low (e.g., <3000 ppm), as an incompatible element it becomes highly enriched (e.g. >10 wt%) in the final few percent of crystallization. In order to understand how a hydrous magma ocean would crystallize at the top of the lower mantle, we determined liquidus phase relations in the MgO-FeO-CaO-Al2O3-SiO2-H2O system at 24 GPa. We find that the bridgmanite (brg) + stishovite (st) + melt and bridgmanite (brg) + ferropericlase (fp) + melt cotectic boundary curves trend to Mg-rich melt compositions with decreasing temperature and extend to very high H2O contents (similar to 80 mol% H2O). The brg+st+melt curve is a subtraction curve at < similar to 18 mol% H2O and a reaction curve at higher H2O contents, whereas the brg+fp+melt is a subtraction curve throughout its length. The density of melts along the two cotectics leads to neutral buoyancy with respect to shallow lower mantle and transition zone minerals at H2O contents up to similar to 25 mol%. A transient melt-rich layer can form at the top of the lower mantle during late-stage crystallization in a mushy magma ocean when melt percolation dominates. When crystallization exceeds similar to 98%, hydrous melts (>25 mol% H2O) become buoyant and can percolate into and hydrate the mantle transition zone.

With the advent of toroidal and double-stage diamond anvil cells (DACs), pressures between 4 and 10 Mbar can be achieved under static compression, however, the ability to explore diverse sample assemblies is limited on these micron-scale anvils. Adapting the toroidal DAC to support larger sample volumes offers expanded capabilities in physics, chemistry, and planetary science: including, characterizing materials in soft pressure media to multi-megabar pressures, synthesizing novel phases, and probing planetary assemblages at the interior pressures and temperatures of super-Earths and sub-Neptunes. Here we have continued the exploration of larger toroidal DAC profiles by iteratively testing various torus and shoulder depths with central culet diameters in the 30-50m range. We present a 30m culet profile that reached a maximum pressure of 414(1) GPa based on a Pt scale. The 300K equations of state fit to our P-V data collected on gold and rhenium are compatible with extrapolated hydrostatic equations of state within 1% up to 4 Mbar. This work validates the performance of these large-culet toroidal anvils to>4 Mbar and provides a promising foundation to develop toroidal DACs for diverse sample loading and laser heating.

The tip of the red giant branch (TRGB) based distance method in the I band is one of the most efficient and precise techniques for measuring distances to nearby galaxies (D less than or similar to 15 Mpc). The TRGB in the near-infrared (NIR) is 1-2 mag brighter relative to the I band, and has the potential to expand the range over which distance measurements to nearby galaxies are feasible. Using Hubble Space Telescope (HST) imaging of 12 fields in eight nearby galaxies, we determine color-based corrections and zero-points of the TRGB in the Wide Field Camera 3 IR (WFC3/IR) F110W and F160W filters. First, we measure TRGB distances in the I band equivalent Advanced Camera System (ACS) F814W filter from resolved stellar populations with the HST. The TRGB in the ACS F814W filter is used for our distance anchor and to place the WFC3/IR magnitudes on an absolute scale. We then determine the color dependence (a proxy for metallicity/age) and zero-point of the NIR TRGB from photometry of WFC3/IR fields that overlap with the ACS fields. The new calibration is accurate to similar to 1% in distance relative to the F814W TRGB. Validating the accuracy of the calibrations, we find that the distance modulus for each field using the NIR TRGB calibration agrees with the distance modulus of the same field as determined from the F814W TRGB. This is a JWST preparatory program, and the work done here will directly inform our approach to calibrating the TRGB in JWST NIRCam and NIRISS photometric filters.

Protoplanetary disks are often assumed to change slowly and smoothly during planet formation. Here, we investigate the time evolution of isolated disks subject to viscosity and a disk wind. The viscosity is assumed to increase rapidly at around 900 K due to thermal ionization of alkali metals, or thermionic and ion emission from dust, and the onset of magnetorotational instability (MRI). The disks generally undergo large, rapid fluctuations for a wide range of time-averaged mass accretion rates. Fluctuations involve coupled waves in temperature and surface density that move radially in either direction through the inner 1.5 au of the disk. Two types of waves are seen with radial speeds of roughly 50 and 1000 cm s-1, respectively. The pattern of waves repeats with a period of roughly 10,000 yr that depends weakly on the average mass accretion rate. Viscous transport due to MRI is confined to the inner disk. This region is resupplied by mass flux from the outer disk driven by the disk wind. Interior to 1 au, the temperature and surface density can vary by a factor of 2-10 on timescales of years to kiloyears. The stellar mass accretion rate varies by 3 orders of magnitude on a similar timescale. This behavior lasts for at least 1 Myr for initial disks comparable to the minimum-mass solar nebula.

Ethylene plays its essential roles in plant development, growth, and defense responses by controlling the transcriptional reprograming, in which EIN2-C-directed regulation of histone acetylation is the first key -step for chromatin to perceive ethylene signaling. But how the nuclear acetyl coenzyme A (acetyl CoA) is produced to ensure the ethylene -mediated histone acetylation is unknown. Here we report that ethylene triggers the accumulation of the pyruvate dehydrogenase complex (PDC) in the nucleus to synthesize nuclear acetyl CoA to regulate ethylene response. PDC is identified as an EIN2-C nuclear partner, and ethylene triggers its nuclear accumulation. Mutations in PDC lead to an ethylene-hyposensitivity that results from the reduction of histone acetylation and transcription activation. Enzymatically active nuclear PDC synthesize nuclear acetyl CoA for EIN2-C-directed histone acetylation and transcription regulation. These findings uncover a mechanism by which PDC-EIN2 converges the mitochondrial enzyme mediated nuclear acetyl CoA synthesis with epigenetic and transcriptional regulation for plant hormone response.

Animal regeneration involves coordinated responses across cell types throughout the animal body. In endosymbiotic animals, whether and how symbionts react to host injury and how cellular responses are integrated across species remain unexplored. Here, we study the acoel Convolutriloba longifissura, which hosts symbiotic Tetraselmis sp. green algae and can regenerate entire bodies from tissue fragments. We show that animal injury causes a decline in the photosynthetic efficiency of the symbiotic algae, alongside two distinct, sequential waves of transcriptional responses in acoel and algal cells. The initial algal response is characterized by the upregulation of a cohort of photosynthesis-related genes, though photosynthesis is not necessary for regeneration. A conserved animal transcription factor, runt, is induced after injury and required for acoel regeneration. Knockdown of Cl-runt dampens transcriptional responses in both species and further reduces algal photosynthetic efficiency post-injury. Our results suggest that the holobiont functions as an integrated unit of biological organization by coordinating molecular networks across species through the runt-dependent animal regeneration program.

Background In dinoflagellates, a unique and extremely divergent genomic and nuclear organization has evolved. The highly unusual features of dinoflagellate nuclei and genomes include permanently condensed liquid crystalline chromosomes, primarily packaged by proteins other than histones, genes organized in very long unidirectional gene arrays, a general absence of transcriptional regulation, high abundance of the otherwise very rare DNA modification 5-hydroxymethyluracil (5-hmU), and many others. While most of these fascinating properties are originally identified in the 1970s and 1980s, they have not yet been investigated using modern genomic tools. Results In this work, we address some of the outstanding questions regarding dinoflagellate genome organization by mapping the genome-wide distribution of 5-hmU (using both immunoprecipitation-based and basepair-resolution chemical mapping approaches) and of chromatin accessibility in the genome of the Symbiodiniaceae dinoflagellate Breviolum minutum. We find that the 5-hmU modification is preferentially enriched over certain classes of repetitive elements, often coincides with the boundaries between gene arrays, and is generally correlated with decreased chromatin accessibility, the latter otherwise being largely uniform along the genome. We discuss the potential roles of 5-hmU in the functional organization of dinoflagellate genomes and its relationship to the transcriptional landscape of gene arrays. Conclusions Our results provide the first window into the 5-hmU and chromatin accessibility landscapes in dinoflagellates.

Microalgae contribute to about half of global net photosynthesis, which converts sunlight into the chemical energy (ATP and NADPH) used to transform CO2 into biomass. Alternative electron pathways of photosynthesis have been proposed to generate additional ATP that is required to sustain CO2 fixation. However, the relative importance of each alternative pathway remains elusive. Here, we dissect and quantify the contribution of cyclic, pseudo-cyclic and chloroplast-to-mitochondria electron flows for their ability to sustain net photosynthesis in the microalga Chlamydomonas reinhardtii. We show that (i) each alternative pathway can provide sufficient additional energy to sustain high CO2 fixation rates, (ii) the alternative pathways exhibit cross-compensation, and (iii) the activity of at least one of the three alternative pathways is necessary to sustain photosynthesis. We further show that all pathways have very different efficiencies at energizing CO2 fixation, with the chloroplast-mitochondria interaction being the most efficient. Overall, our data lay bioenergetic foundations for biotechnological strategies to improve CO2 capture and fixation.

The thylakoid membrane lipid sulfoquinovosyldiacylglycerol (SQDG) is an anionic molecule that functions in stabilizing the thylakoid membranes and photosystem (PS) supercomplexes. In this study, we characterized the function of GreenCut protein CrLPB1, which encodes UDP-glucose pyrophosphorylase (UGP3), an enzyme involved in SQDG biosynthesis. The lpb1 mutants had reduced levels of SQDG, grew more slowly than wild type cells or the complemented strain under photoautotrophic conditions and were impacted in its rate of oxygen evolution and photosystem II (PSII) activity, especially electron transfer from Q(A)(-) to Q(B). Furthermore, the structure of the PSII supercomplex and morphology of the thylakoid membranes were also both altered in lpb1. In conclusion, LPB1 is involved in SQDG biosynthesis, which in turn appears to be critical in maintaining normal thylakoid membrane structure and PSII activity/stability.