The gut is continuously invaded by diverse bacteria from the diet and the environment, yet microbiome composition is relatively stable over time for host species ranging from mammals to insects, suggesting host-specific factors may selectively maintain key species of bacteria. To investigate host specificity, we used gnotobiotic Drosophila, microbial pulse-chase protocols, and microscopy to investigate the stability of different strains of bacteria in the fly gut. We show that a host-constructed physical niche in the foregut selectively binds bacteria with strain-level specificity, stabilizing their colonization. Primary colonizers saturate the niche and exclude secondary colonizers of the same strain, but initial colonization by Lactobacillus species physically remodels the niche through production of a glycan-rich secretion to favor secondary colonization by unrelated commensals in the Acetobacter genus. Our results provide a mechanistic framework for understanding the establishment and stability of a multi-species intestinal microbiome.

The "new core paradox" suggests that the persistence of the geomagnetic field over nearly all of Earth history is in conflict with the core being highly thermally conductive, which makes convection and dynamo action in the core much harder prior to the nucleation of the inner core. Here we revisit this issue by exploring the influence of six important parameters on core evolution: upper/lower mantle viscosity ratio, core thermal conductivity, core radiogenic heat rate, mantle radiogenic heating rate, central core melting temperature, and initial core-mantle boundary (CMB) temperature. Each parameter is systematically explored by the model, which couples mantle energy and core energy-entropy evolution. A model is successful if the correct present-day inner core size is achieved and the dynamo remains alive, as implied by the paleomagnetic record. In agreement with previous studies, we do not find successful thermal evolutions using nominal parameters, which includes a core thermal conductivity of 70 Wm(-1)K(-1), zero core radioactivity, and an initial CMB temperature of 5,000 K. The dynamo can be kept alive by assuming an unrealistically low thermal conductivity of 20 Wm(-1)K(-1) or an unrealistically high core radioactive heat flow of 3 TW at present-day, which are considered "unsuccessful" models. We identify a third scenario to keep the dynamo alive by assuming a hot initial CMB temperature of similar to 6,000 K and a central core liquidus of similar to 5,550 K. These temperatures are on the extreme end of typical estimates, but should not be ruled out and deserve further scrutiny.

Future direct imaging missions similar to the HabEx and LUVOIR mission concepts aim to catalog and characterize Earth-mass analogs around nearby stars. The exoplanet yield of these missions will be dependent on the frequency of Earth-like planets, and potentially the a priori knowledge of which stars specifically host suitable planetary systems. Ground- or space-based radial velocity surveys can potentially perform the pre-selection of targets and assist in the optimization of observation times, as opposed to an uninformed direct imaging survey. In this paper, we present our framework for simulating future radial velocity surveys of nearby stars in support of direct imaging missions. We generate lists of exposure times, observation time-series, and radial velocity time-series given a direct imaging target list. We generate simulated surveys for a proposed set of telescopes and precise radial velocity spectrographs spanning a set of plausible global-network architectures that may be considered for next-generation extremely precise radial velocity surveys. We also develop figures of merit for observation frequency and planet detection sensitivity, and compare these across architectures. From these, we draw conclusions, given our stated assumptions and caveats, to optimize the yield of future radial velocity surveys supporting direct imaging missions. We find that all of our considered surveys obtain sufficient numbers of precise observations to meet the minimum theoretical white noise detection sensitivity for Earth-mass habitable-zone planets. While our detection rates and mass-sensitivity are optimistic, we have margin to explore systematic effects due to stellar activity and correlated noise in future work.

Compared with conventional, solution-phase approaches, solid-state reaction methods can provide unique access to novel synthetic targets. Nanothreads-one-dimensional diamondoid polymers formed through the compression of small molecules-represent a new class of materials produced via solid-state reactions, however, the formation of chemically homogeneous products with targeted functionalization represents a persistent challenge. Through careful consideration of molecular precursor stacking geometry and functionalization, we report here the scalable synthesis of chemically homogeneous, functionalized nanothreads through the solid-state polymerization of 2,5-furandicarboxylic acid. The resulting product possesses high-density, pendant carboxyl functionalization along both sides of the backbone, enabling new opportunities for the post-synthetic processing and chemical modification of nanothread materials applicable to a broad range of potential applications.

The cellular mechanisms responsible for the regulation of nutrient exchange, immune response, and symbiont population growth in the cnidarian-dinoflagellate symbiosis are poorly resolved. Here, we employed liquid chromatography-mass spectrometry to elucidate proteomic changes associated with symbiosis in Breviolum minutum, a native symbiont of the sea anemone Exaiptasia diaphana ('Aiptasia'). We manipulated nutrients available to the algae in culture and to the holobiont in hospite (i.e., in symbiosis) and then monitored the impacts of our treatments on host-endosymbiont interactions. Both the symbiotic and nutritional states had significant impacts on the B. minutum proteome. B. minutum in hospite showed an increased abundance of proteins involved in phosphoinositol metabolism (e.g., glycerophosphoinositol permease 1 and phosphatidylinositol phosphatase) relative to the free-living alga, potentially reflecting inter-partner signalling that promotes the stability of the symbiosis. Proteins potentially involved in concentrating and fixing inorganic carbon (e.g., carbonic anhydrase, V-type ATPase) and in the assimilation of nitrogen (e.g., glutamine synthase) were more abundant in free-living B. minutum than in hospite, possibly due to host-facilitated access to inorganic carbon and nitrogen limitation by the host when in hospite. Photosystem proteins increased in abundance at high nutrient levels irrespective of the symbiotic state, as did proteins involved in antioxidant defences (e.g., superoxide dismutase, glutathione s-transferase). Proteins involved in iron metabolism were also affected by the nutritional state, with an increased iron demand and uptake under low nutrient treatments. These results detail the changes in symbiont physiology in response to the host microenvironment and nutrient availability and indicate potential symbiont-driven mechanisms that regulate the cnidarian-dinoflagellate symbiosis.