Lactobacilli and Acetobacter sp. are commercially important bacteria that often form communities in natural fermentations, including food preparations, spoilage, and in the digestive tract of the fruit fly Drosophila melanogaster. Communities of these bacteria are widespread and prolific, despite numerous strain-specific auxotrophies, suggesting they have evolved nutrient interdependencies that regulate their growth. The use of a chemically-defined medium (CDM) supporting the growth of both groups of bacteria would facilitate the identification of the molecular mechanisms for the metabolic interactions between them. While numerous CDMs have been developed that support specific strains of lactobacilli or Acetobacter, there has not been a medium formulated to support both genera. We developed such a medium, based on a previous CDM designed for growth of lactobacilli, by modifying the nutrient abundances to improve growth yield. We further simplified the medium by substituting casamino acids in place of individual amino acids and the standard Wolfe's vitamins and mineral stocks in place of individual vitamins and minerals, resulting in a reduction from 40 to 8 stock solutions. These stock solutions can be used to prepare several CDM formulations that support robust growth of numerous lactobacilli and Acetobacters. Here, we provide the composition and several examples of its use, which is important for tractability in dissecting the genetic and metabolic basis of natural bacterial species interactions.

Studies of material returned from Cb asteroid Ryugu have revealed considerable mineralogical and chemical heterogeneity, stemming primarily from brecciation and aqueous alteration. Isotopic anomalies could have also been affected by delivery of exogenous clasts and aqueous mobilization of soluble elements. Here, we show that isotopic anomalies for mildly soluble Cr are highly variable in Ryugu and CI chondrites, whereas those of Ti are relatively uniform. This variation in Cr isotope ratios is most likely due to physicochemical fractionation between 54Cr-rich presolar nanoparticles and Cr-bearing secondary minerals at the millimeter-scale in the bulk samples, likely due to extensive aqueous alteration in their parent bodies that occurred [Formula: see text] after Solar System birth. In contrast, Ti isotopes were marginally affected by this process. Our results show that isotopic heterogeneities in asteroids are not all nebular or accretionary in nature but can also reflect element redistribution by water.

We have discovered two epochs of activity on quasi-Hilda 2009 DQ118. Small bodies that display comet-like activity, such as active asteroids and active quasi-Hildas, are important for understanding the distribution of water and other volatiles throughout the solar system. Through our NASA Partner Citizen Science project, Active Asteroids, volunteers classified archival images of 2009 DQ118 as displaying comet-like activity. By performing an in-depth archival image search, we found over 20 images from UT 2016 March 8-9 with clear signs of a comet-like tail. We then carried out follow-up observations of 2009 DQ118 using the 3.5 m Astrophysical Research Consortium Telescope at Apache Point Observatory, Sunspot, New Mexico, USA and the 6.5 m Magellan Baade Telescope at Las Campanas Observatory, Chile. These images revealed a second epoch of activity associated with the UT 2023 April 22 perihelion passage of 2009 DQ118. We performed photometric analysis of the tail and find that it had a similar apparent length and surface brightness during both epochs. We also explored the orbital history and future of 2009 DQ118 through dynamical simulations. These simulations show that 2009 DQ118 is currently a quasi-Hilda and that it frequently experiences close encounters with Jupiter. We find that 2009 DQ118 is currently on the boundary between asteroidal and cometary orbits. Additionally, it has likely been a Jupiter family comet or Centaur for much of the past 10 kyr and will be in these same regions for the majority of the next 10 kyr. Since both detected epochs of activity occurred near perihelion, the observed activity is consistent with sublimation of volatile ices. 2009 DQ118 is currently observable until similar to mid-October 2023. Further observations would help to characterize the observed activity.

Protein-protein interactions play a crucial role in driving cellular processes and enabling appropriate physiological responses in organisms. The plant hormone ethylene signaling pathway is complex and regulated by the spatiotemporal regulation of its signaling molecules. Constitutive Triple Response 1 (CTR1), a key negative regulator of the pathway, regulates the function of Ethylene-Insensitive 2 (EIN2), a positive regulator of ethylene signaling, at the endoplasmic reticulum (ER) through phosphorylation. Our recent study revealed that CTR1 can also translocate from the ER to the nucleus in response to ethylene and positively regulate ethylene responses by stabilizing EIN3. To gain further insights into the role of CTR1 in plants, we used TurboID-based proximity labeling and mass spectrometry to identify the proximal proteomes of CTR1 in Nicotiana benthamiana. The identified proximal proteins include known ethylene signaling components, as well as proteins involved in diverse cellular processes such as mitochondrial respiration, mRNA metabolism, and organelle biogenesis. Our study demonstrates the feasibility of proximity labeling using the N. benthamiana transient expression system and identifies the potential interactors of CTR1 in vivo, uncovering the potential roles of CTR1 in a wide range of cellular processes.

Plant cell expansion is driven by turgor pressure and regulated by hormones. How plant cells avoid cell wall rupture during hormone-induced cell expansion remains a mystery. Here we show that brassinosteroid (BR), while stimulating cell elongation, promotes the plasma membrane (PM) accumulation of the receptor kinase FERONIA (FER), which monitors cell wall damage and in turn attenuates BR-induced cell elongation to prevent cell rupture. The GSK3-like kinase BIN2 phosphorylates FER, resulting in reduced FER accumulation and translocation from endoplasmic reticulum to PM. By inactivating BIN2, BR signaling promotes dephosphorylation and increases PM accumulation of FER, thereby enhancing the surveillance of cell wall integrity. Our study reveals a vital signaling circuit that coordinates hormone signaling with mechanical sensing to prevent cell bursting during hormone-induced cell expansion.

For the first time on Mars, the crystalline magnesium-sulfate mineral starkeyite (MgSO4 center dot 4H(2)O) was definitively identified using the CheMin X-ray diffraction instrument at Gale crater. At the Canaima drill site, starkeyite along with amorphous MgSO4 center dot nH(2)O are among the "polyhydrated Mg-sulfates" interpreted in orbital reflectance spectra. Mg-sulfates are good climate indicators as they are very responsive to changes in temperature and relative humidity. We hypothesize that, through evaporation, Mg-sulfates formed at the end of brine evolution when ion concentrations became saturated and precipitated on the surface or near sub-surface as either epsomite or meridianiite. These minerals were subsequently dehydrated later to starkeyite and amorphous MgSO4 center dot nH(2)O in response to a drier Mars. At Canaima, starkeyite is stable and would form during the warmer Mars summers. Due to very slow kinetics at the low Mars winter temperatures, starkeyite and amorphous MgSO4 center dot nH(2)O would be resistant to recrystallize to more hydrous forms and thus likely persist year-round. During the course of analyses, starkeyite transforms into amorphous MgSO4 center dot nH(2)O inside the rover body due to the elevated temperature and greatly reduced relative humidity compared to the martian surface at the Canaima drill site. It is possible that crystalline sulfate minerals existed in earlier samples measured by CheMin but altered inside the rover before they could be analyzed. Starkeyite is most likely prevalent in the subsurface, whereas both starkeyite and amorphous MgSO4 center dot nH(2)O are likely present on the surface as starkeyite could partially transform into amorphous MgSO4 center dot nH(2)O due to direct solar heating.

Intrinsically disordered proteins (IDP) lack stable tertiary structures, which allows them to change conformation and function under different physicochemical conditions. This may be highly advantageous for plants, which often use changes in their environment to elicit a variety of responses, including developmental events. For instance, some plants delay flowering in the fall and require exposure to winter temperatures as a cue to initiate flowering the following spring. Many of the genes involved in temperature-dependent flowering have been extensively studied in Arabidopsis, yet how plants coordinate their molecular states with seasonal temperature change is poorly understood. Here, we explore the role of temperature-sensitive phase separation of the IDP and flowering-time regulator, SUPPRESSOR OF FRIGIDA 4 (SUF4), in modulating flowering time. SUF4 has a well-defined role in regulating temperature-dependent flowering time by activating the master floral suppressor FLOWERING LOCUS C (FLC). We show that in plant nuclei, SUF4 is a temperature-sensitive protein that assembles into biomolecular condensates in warm temperatures (20{degrees}C). When temperatures cool (4{degrees}C), SUF4 nuclear condensates disassemble, causing SUF4 to disperse within the nucleoplasm. Additionally, we demonstrate that the number of SUF4 condensates quantitatively correlates with flowering time. Progressive alterations to the amino acid composition of SUF4's disordered region cause likewise progressive changes in temperature-dependent condensation both in vitro and in vivo, FLC transcription, and the onset of flowering. Lastly, we observe that SUF4 condensates coincide with the accumulation of other key flowering-time proteins (FRIGIDA and ELF7). These findings indicate that condensation of SUF4 likely plays a pivotal role in promoting flowering, possibly by concentrating and stabilizing regulatory factors needed for the transcriptional activation of FLC through temperature-dependent phase separation. This research suggests that in plants, IDPs can sense environmental cues and regulate critical developmental processes.

We report the discovery and Doppler mass measurement of a 7.4 days 2.3 R circle plus mini-Neptune around a metal-poor K dwarf BD+29 2654 (TOI-2018). Based on a high-resolution Keck/HIRES spectrum, the Gaia parallax, and multiwavelength photometry from the UV to the mid-infrared, we found that the host star has = T-eff 4174(-42)(+34) K, log g (4.62) (-0.03) = + 0.02, [Fe/H] = - 0.58 +/- 0.18, M-* = 0.57 +/- 0.02 Me, and R-* = 0.62 +/- 0.01 R-?. Precise Doppler measurements with Keck/HIRES revealed a planetary mass of M-p = 9.2 +/- 2.1 M-? for TOI-2018 b. TOI-2018 b has a mass and radius that are consistent with an Earthlike core, with a similar to 1%-by-mass hydrogen/helium envelope or an ice-rock mixture. The mass of TOI-2018 b is close to the threshold for runaway accretion and hence giant planet formation. Such a threshold is predicted to be around 10M(circle plus) or lower for a low-metallicity (low-opacity) environment. If TOI-2018 b is a planetary core that failed to undergo runaway accretion, it may underline the reason why giant planets are rare around low-metallicity host stars (one possibility is their shorter disk lifetimes). With a K-band magnitude of 7.1, TOI-2018 b may be a suitable target for transmission spectroscopy with the James Webb Space Telescope. The system is also amenable to metastable Helium observation; the detection of a Helium exosphere would help distinguish between a H/He-enveloped planet and a water world.