NWA 8785 is a remarkable EL3 chondrite with a high abundance (similar to 34 vol%) of an Fe-rich matrix. This is the highest matrix abundance known among enstatite chondrites (ECs) and more similar to the matrix abundances in some carbonaceous and Rumuruti chondrites. X-ray diffraction and TEM data indicate that the fine-grained portion of the NWA 8785 matrix consists of nanoscale magnetite mixed with a noncrystalline silicate material and submicron-sized enstatite and plagioclase grains. This is the first report of magnetite nanoparticles in an EL3. The Si content of the metal (0.7 wt%), presence of ferroan alabandite, and its O isotopic composition indicate NWA 8785 is EL3-related. Having more abundant matrix than in other ECs, and that the matrix is rich in magnetite nanoparticles, which are not present in any other EC, suggest classification as an EL3 anomalous. Although we cannot completely exclude any of the mechanisms or environments for formation of the magnetite, we find a secondary origin to be the most compelling. We suggest that the magnetite formed due to hydrothermal activity in the meteorite parent body. Although ECs are relatively dry and likely formed within the nebular snow line, ices may have drifted inward from just beyond the snow line to the region where the EL chondrites were accreting, or more likely the snow line migrated inward during the early evolution of the solar system. This may have resulted in the condensation of ices and provided an ice-rich region for accretion of the EL3 parent body. Thus, the EL3 parent body may have had hydrothermal activity and if Earth formed near the EC accretion zone, similar bodies may have contributed to the Earth's water supply. NWA 8785 greatly extends the range of known characteristics of ECs and EC parent body processes.

Volcano geodesy often involves the use of models to explain observed surface deformation. A variety of forward models are used, from analytical point sources to numerical simulations that consider complex magma system geometries, topography, and material properties. Various inversion methods can then be used to relate observed volcano data to models. Ideally, forward models should be verified through intercomparison, to check for implementation errors and quantify the error induced by any approximations used. Additionally, forward models and inversion methods should be validated through tests with synthetic and/or real data, to determine their ability to match data and estimate parameter values within uncertainty. However, to date, there have not been comprehensive verification and validation efforts in volcano geodesy. Here, we report on the first phase of the Drivers of Volcano Deformation (DVD) exercises, which were designed to build community involvement through web-based exercises involving calculations of static elastic displacement around pressurized magma reservoirs. The forward model exercises begin with a spherical reservoir in a homogeneous half space, then introduce topography, heterogeneous elastic properties, and spheroidal geometries. The inversion exercises provide synthetic noisy surface displacement data for a spherical reservoir in a homogeneous half space and assess consistency in estimates of reservoir location and volume/pressure change. There is variability in the results from both forward modeling and inversions, which highlights the strengths and limitations of different forward models, as well as the importance of inversion method choice and uncertainty quantification. This first phase of the DVD exercises serves as a community resource and will facilitate further efforts to develop standards of reproducibility.

This study analyzed samples of the Murchison and Sutter's Mill carbonaceous chondrite meteorites in support of the future analysis of samples returned from the asteroid (10155) Bennu by the OSIRIS-REx (Origins, Spectral Interpretation, Resource Identification, and Security-Regolith Explorer) mission. Focusing specifically on the insoluble organic matter (IOM), this study establishes that a total of 1.3 g of bulk sample from a single chondritic meteorite are sufficient to obtain a wide range of cosmochemical information, including light element analysis (H, C, and N), isotopic analysis (D/H, 13C/12C, and 15N/14N), and x-ray fluorescence spectroscopy for major elemental abundances. IOM isolated from the bulk meteorite samples was analyzed by light element and isotopic analysis as described above, 1H and 13C solid-state nuclear magnetic resonance spectroscopy, Raman spectroscopy, and complete noble gas analyses (abundances and isotopes). The samples studied included a pair from Murchison (CM2), one of which had been irradiated with high-energy x-rays in the course of computed tomographic imaging. No differences between the irradiated and non-irradiated Murchison samples were observed in the many different chemical and spectroscopic analyses, indicating that any x-ray-derived sample damage is below levels of detection. Elemental, isotopic, and molecular spectroscopic data derived from IOM isolated from the Sutter's Mill sample reveals evidence that this meteorite falls into the class of heated CM chondrites.

Molecular tool development in traditionally non-tractable animals opens new avenues to study gene functions in the relevant ecological context. Entomopathogenic nematodes (EPN) Steinernema and their symbiotic bacteria of Xenorhabdus spp are a valuable experimental system in the laboratory and are applicable in the field to promote agricultural productivity. The infective juvenile (IJ) stage of the nematode packages mutualistic symbiotic bacteria in the intestinal pocket and invades insects that are agricultural pests. The lack of consistent and heritable genetics tools in EPN targeted mutagenesis severely restricted the study of molecular mechanisms underlying both parasitic and mutualistic interactions. Here, I report a protocol for CRISPR-Cas9 based genome-editing that is successful in two EPN species, S. carpocapsae and S. hermaphroditum. I adapted a gonadal microinjection technique in S. carpocapsae, which created on-target modifications of a homologue Sc-dpy-10 (cuticular collagen) by homology directed repair. A similar delivery approach was used to introduce various alleles in S. hermaphroditum including Sh-dpy-10 and Sh-unc-22 (a muscle gene), resulting in visible and heritable phenotypes of dumpy and twitching, respectively. Using conditionally dominant alleles of Sh-unc-22 as a co-CRISPR marker, I successfully modified a second locus encoding Sh-Daf22 (a homologue of human sterol carrier protein SCPx), predicted to function as a core enzyme in the biosynthesis of nematode pheromone that is required for IJ development. As a proof of concept, Sh-daf-22 null mutant showed IJ developmental defects in vivo (in insecta). This research demonstrates that Steinernema spp are highly tractable for targeted mutagenesis and has great potential in the study of gene functions under controlled laboratory conditions within the relevant context of its ecological niche.