Chloroflexus sp. MS-CIW-1 was isolated from a phototrophic mat in Mushroom Spring, an alkaline hot spring in Yellowstone National Park, WY, USA. We report the draft genome of 4.8 Mb consisting of 6 contigs with 3755 protein-coding genes and a GC content of 54.45%.

Mass coral bleaching is one of the clearest threats of climate change to the persistence of marine biodiversity. Despite the negative impacts of bleaching on coral health and survival, some corals may be able to rapidly adapt to warming ocean temperatures. Thus, a significant focus in coral research is identifying the genes and pathways underlying coral heat adaptation. Here, we review state-of-the-art methods that may enable the discovery of heat-adaptive loci in corals and identify four main knowledge gaps. To fill these gaps, we describe an experimental approach combining seascape genomics with CRISPR/Cas9 gene editing to discover and validate heat-adaptive loci. Finally, we discuss how information on adaptive genotypes could be used in coral reef conservation and management strategies.

Anaerobic digestion is a bioenergy technology that can play a vital role in achieving net-zero emissions by converting organic matter into biomethane and biogenic carbon dioxide. By implementing bioenergy with carbon capture and storage (BECCS), carbon dioxide can be separated from biomethane, captured, and permanently stored, thus generating carbon dioxide removal (CDR) to offset hard-to-abate emissions. Here, we quantify the global availability of waste biomass for BECCS and their CDR and biomethane technical potentials. These biomass feedstocks do not create additional impacts on land, water, and biodiversity and can allow a more sustainable development of BECCS while still preserving soil fertility. We find that up to 1.5 Gt CO2 per year, or 3% of global GHG emissions, are available to be deployed for CDR worldwide. The conversion of waste biomass can generate up to 10 700 TWh of bioenergy per year, equivalent to 10% of global final energy consumption and 27% of global natural gas supply. Our assessment quantifies the climate mitigation potential of waste biomass and its capacity to contribute to negative emissions without relying on extensive biomass plantations.

Trait differences between invasive plants and the plants in their recipient communities moderate the impact of invaders on community composition. Callery pear (Pyrus calleryana Decne.) is a fast-growing, stress-tolerant tree native to China that has been widely planted for its ornamental value. In recent decades, P. calleryana has naturalized throughout the eastern United States, where it spreads rapidly and achieves high abundance in early-successional environments. Here we compare the impacts of low-density, establishment-phase P. calleryana to those of functionally similar native trees on the understory community diversity and total cover of three early-successional meadows in Indiana's Eastern Corn Belt Plains. In contrast to our prediction that P. calleryana would have greater negative effects on the total abundance and diversity of the understory plant community compared with native tuliptree (Liriodendron tulipifera L.), American sycamore (Platanus occidentalis L.), or non-tree control plots, we found that these low-density populations of P. calleryana had no significant impact on total cover, species richness, or diversity indices for the understory community compared with the native trees and non-tree control plots. Likewise, the studied populations of P. calleryana had no significant impact on the native, introduced, woody, or native tree subsets of the understory community. These results indicate that in young, low-density populations situated in early-successional meadows, the trait differences between P. calleryana and functionally similar native trees are not of a great enough magnitude to produce changes in community composition. Going forward, complementary research on the impacts of P. calleryana on community composition and ecosystem processes in areas with long-established, dense invasions or invasions in more sensitive ecosystems would allow us to more fully understand how this widespread invader disrupts its host ecosystems.

We present a spectroscopic analysis of Eridanus IV (Eri IV) and Centaurus I (Cen I), two ultrafaint dwarf galaxies of the Milky Way. Using IMACS/Magellan spectroscopy, we identify 28 member stars of Eri IV and 34 member stars of Cen I. For Eri IV, we measure a systemic velocity of vsys=-31.5-1.2+1.3kms-1 , and velocity dispersion sigma v=6.1-0.9+1.2kms-1 . Additionally, we measure the metallicities of 16 member stars of Eri IV. We find a metallicity of [Fe/H]=-2.87-0.07+0.08 , and resolve a dispersion of sigma [Fe/H]=0.20 +/- 0.09. The mean metallicity is marginally lower than all other known ultrafaint dwarf galaxies, making it one of the most metal-poor galaxies discovered thus far. Eri IV also has a somewhat unusual right-skewed metallicity distribution. For Cen I, we find a velocity v sys = 44.9 +/- 0.8 km s-1, and velocity dispersion sigma v=4.2-0.5+0.6kms-1 . We measure the metallicities of 27 member stars of Cen I, and find a mean metallicity [Fe/H] = -2.57 +/- 0.08, and metallicity dispersion sigma[Fe/H]=0.38-0.05+0.07 . We calculate the systemic proper motion, orbit, and the astrophysical J-factor for each system, the latter of which indicates that Eri IV is a good target for indirect dark matter detection. We also find no strong evidence for tidal stripping of Cen I or Eri IV. Overall, our measurements confirm that Eri IV and Cen I are dark-matter-dominated galaxies with properties largely consistent with other known ultrafaint dwarf galaxies. The low metallicity, right-skewed metallicity distribution, and high J-factor make Eri IV an especially interesting candidate for further follow-up.

We present high-cadence ultraviolet through near-infrared observations of the Type Ia supernova (SN Ia) 2023bee at D = 32 +/- 3 Mpc, finding excess flux in the first days after explosion, particularly in our 10 minutes cadence TESS light curve and Swift UV data. Compared to a few other normal SNe Ia with early excess flux, the excess flux in SN 2023bee is redder in the UV and less luminous. We present optical spectra of SN 2023bee, including two spectra during the period where the flux excess is dominant. At this time, the spectra are similar to those of other SNe Ia but with weaker Si ii, C ii, and Ca ii absorption lines, perhaps because the excess flux creates a stronger continuum. We compare the data to several theoretical models on the origin of early excess flux in SNe Ia. Interaction with either the companion star or close-in circumstellar material is expected to produce a faster evolution than observed. Radioactive material in the outer layers of the ejecta, either from double detonation explosion or from a 56Ni clump near the surface, cannot fully reproduce the evolution either, likely due to the sensitivity of early UV observable to the treatment of the outer part of ejecta in simulation. We conclude that no current model can adequately explain the full set of observations. We find that a relatively large fraction of nearby, bright SNe Ia with high-cadence observations have some amount of excess flux within a few days of explosion. Considering potential asymmetric emission, the physical cause of this excess flux may be ubiquitous in normal SNe Ia.