After eight months of continuous observations, the Wide-field Infrared Survey Explorer (WISE) mapped the entire sky at 3.4 mu m, 4.6 mu m, 12 mu m, and 22 mu m. We have begun a dedicated WISE High Resolution Galaxy Atlas project to fully characterize large, nearby galaxies and produce a legacy image atlas and source catalog. Here we summarize the deconvolution techniques used to significantly improve the spatial resolution of WISE imaging, specifically designed to study the internal anatomy of nearby galaxies. As a case study, we present results for the galaxy NGC 1566, comparing the WISE enhanced-resolution image processing to that of Spitzer, Galaxy Evolution Explorer, and ground-based imaging. This is the first paper in a two-part series; results for a larger sample of nearby galaxies are presented in the second paper.

The Wide-field Infrared Survey Explorer (WISE) mapped the entire sky at mid-infrared wavelengths 3.4 mu m, 4.6 mu m, 12 mu m, and 22 mu m. The mission was primarily designed to extract point sources, leaving resolved and extended sources, for the most part, unexplored. Accordingly, we have begun a dedicated WISE Enhanced Resolution Galaxy Atlas (WERGA) project to fully characterize large, nearby galaxies and produce a legacy image atlas and source catalog. Here we demonstrate the first results of the WERGA project for a sample of 17 galaxies, chosen to be of large angular size, diverse morphology, and covering a range in color, stellar mass, and star formation. It includes many well-studied galaxies, such as M 51, M 81, M 87, M 83, M 101, and IC 342. Photometry and surface brightness decomposition is carried out after special super-resolution processing, achieving spatial resolutions similar to that of Spitzer Infrared Array Camera. The enhanced resolution method is summarized in the first paper of this two-part series. In this second work, we present WISE, Spitzer, and Galaxy Evolution Explorer (GALEX) photometric and characterization measurements for the sample galaxies, combining the measurements to study the global properties. We derive star formation rates using the polycyclic aromatic hydrocarbon sensitive 12 mu m (W3) fluxes, warm-dust sensitive 22 mu m (W4) fluxes, and young massive-star sensitive ultraviolet (UV) fluxes. Stellar masses are estimated using the 3.4 mu m (W1) and 4.6 mu m (W2) measurements that trace the dominant stellar mass content. We highlight and showcase the detailed results of M 83, comparing the WISE/Spitzer results with the Australia Telescope Compact Array Hi gas distribution and GALEX UV emission, tracing the evolution from gas to stars. In addition to the enhanced images, WISE's all-sky coverage provides a tremendous advantage over Spitzer for building a complete nearby galaxy catalog, tracing both stellar mass and star formation histories. We discuss the construction of a complete mid-infrared catalog of galaxies and its complementary role of studying the assembly and evolution of galaxies in the local universe.

We present Atacama Large Millimeter/submillimeter Array (ALMA) 870 mu m (345 GHz) data for 49 high-redshift (0.47 < z < 2.85), luminous (11.7 < log(L-bol/L-circle dot) < 14.2) radio-powerful active galactic nuclei (AGNs), obtained to constrain cool dust emission from starbursts concurrent with highly obscured radiative-mode black hole (BH) accretion in massive galaxies that possess a small radio jet. The sample was selected from the Wide-field Infrared Survey Explorer with extremely steep (red) mid-infrared colors and with compact radio emission from NVSS/FIRST. Twenty-six sources are detected at 870 mu m, and we find that the sample has large mid- to far-infrared luminosity ratios, consistent with a dominant and highly obscured quasar. The rest-frame 3 GHz radio powers are 24.7 < log P-3.0 GHz/W Hz(-1)) < 27.3, and all sources are radio-intermediate or radio-loud. BH mass estimates are 7.7 < log(M-BH/M-circle dot) < 10.2. The rest-frame 1-5 mu m spectral energy distributions are very similar to the "Hot DOGs" (hot dust-obscured galaxies), and steeper (redder) than almost any other known extragalactic sources. ISM masses estimated for the ALMA-detected sources are 9.9 < log (M-ISM/M-circle dot) < 11.75 assuming a dust temperature of 30 K. The cool dust emission is consistent with star formation rates reaching several thousand M-circle dot yr(-1), depending on the assumed dust temperature, but we cannot rule out the alternative that the AGN powers all the emission in some cases. Our best constrained source has radiative transfer solutions with approximately equal contributions from an obscured AGN and a young (10-15 Myr) compact starburst.

The non-protein amino acid beta-aminobutyric acid (BABA) primes Arabidopsis to respond more quickly and strongly to pathogen and osmotic stress. Here, we report that BABA also significantly enhances acquired thermotolerance in Arabidopsis. This thermotolerance was dependent on heat shock protein 101, a critical component of the normal heat-shock response. BABA did not enhance basal thermotolerance under a severe heat-shock treatment. No roles for the hormones ethylene and salicylic acid in BABA-induced acquired thermotolerance were identified by mutant analysis. Using global gene expression analysis, transcript levels for several transcription factors and DNA binding proteins regulating responses to the stress hormone abscisic acid (ABA) were found to be elevated in BABA-treated plants compared with water-treated plants. The role of ABA in BABA-induced thermotolerance was complex. BABA-enhanced thermotolerance was partially compromised in the ABA-insensitive mutant, abi1-1, but was augmented in abi2-1. In an unrelated process, BABA, like ABA, inhibited root growth, and the level of inhibition was roughly additive in roots treated with both compounds. Root growth of both abi1-1 and abi2-1 was also inhibited by BABA. Unexpectedly, abi1-1 and abi2-1 root growth was inhibited more strongly by combined ABA and BABA treatments than by BABA alone. Our results, together with previously published data, suggest that BABA is a general enhancer of plant stress resistance, and that cross-talk occurs between BABA and ABA signalling cascades. Specifically, the BABA-mediated accumulation of ABA transcription factors without concomitant activation of a downstream ABA response could represent one component of the BABA-primed state in Arabidopsis.

Protein phosphorylation is crucial for the regulation of enzyme activity and gene expression under osmotic condition. Mass spectrometry (MS)-based phosphoproteomics has transformed the way of studying plant signal transduction. However, requirement of lots of starting materials and prolonged MS measurement time to achieve the depth of coverage has been the limiting factor for the high throughput study of global phosphoproteomic changes in plants. To improve the sensitivity and throughput of plant phosphoproteomics, we have developed a stop and go extraction (stage) tip based phosphoproteomics approach coupled with Tandem Mass Tag (TMT) labeling for the rapid and comprehensive analysis of plant phosphorylation perturbation in response to osmotic stress. Leveraging the simplicity and high throughput of stage tip technique, the whole procedure takes approximately one hour using two tips to finish phosphopeptide enrichment, fractionation, and sample cleaning steps, suggesting an easy-to-use and high efficiency of the approach. This approach not only provides an in-depth plant phosphoproteomics analysis (> 11,000 phosphopeptide identification) but also demonstrates the superior separation efficiency (< 5% overlap) between adjacent fractions. Further, multiplexing has been achieved using TMT labeling to quantify the phosphoproteomic changes of wild-type and snrk2 decuple mutant plants. This approach has successfully been used to reveal the phosphorylation events of Raf-like kinases in response to osmotic stress, which sheds light on the understanding of early osmotic signaling in land plants.

We report on observations of redshifted CO(1-0) line emission and observed-frame similar to 30 GHz radio continuum emission from five ultra-luminous, mid-IR selected hot, Dust-Obscured Galaxies (Hot DOGs) at z greater than or similar to 3 using the Karl G. Jansky Very Large Array. We detect CO(1-0) line emission in all five Hot DOGs, with one of them at high signal-to-noise ratio. We analyse FIR-radio spectral energy distributions, including dust, free-free, and synchrotron emission for the galaxies. We find that most of the 115 GHz rest-frame continuum is mostly due to synchrotron or free-free emission, with only a potentially small contribution from thermal emission. We see a deficit in the rest-frame 115 GHz continuum emission compared to dusty star-forming galaxies and sub-millimetre galaxies (SMGs) at high redshift, suggesting that Hot DOGs do not have similar cold gas reserves compared with star-forming galaxies. One target, W2305-0039, is detected in the FIRST 1.4 GHz survey, and is likely to possess compact radio jets. We compare to the FIR-radio correlation, and find that at least half of the Hot DOGs in our sample are radio-quiet with respect to normal galaxies. These findings suggest that Hot DOGs have comparably less cold molecular gas than star-forming galaxies at lower, z similar to 2 redshifts, and are dominated by powerful, yet radio-quiet AGN.

Chewing herbivores activate plant defense responses through a combination of mechanical wounding and elicitation by herbivore-associated molecular patterns (HAMPs). HAMPs are wound response amplifiers; however, specific defense outputs may also exist that strictly require HAMP-mediated defense signaling. To investigate HAMP-mediated signaling and defense responses, we characterized cowpea (Vigna unguiculata) transcriptome changes following elicitation by inceptin, a peptide HAMP common in Lepidoptera larvae oral secretions. Following inceptin treatment, we observed large-scale reprogramming of the transcriptome consistent with three different response categories: (i) amplification of mechanical wound responses, (ii) temporal extension through accelerated or prolonged responses, and (iii) examples of inceptin-specific elicitation and suppression. At both early and late timepoints, namely 1 and 6 h, large sets of transcripts specifically accumulated following inceptin elicitation. Further early inceptin-regulated transcripts were classified as reversing changes induced by wounding alone. Within key signaling- and defense-related gene families, inceptin-elicited responses included target subsets of wound-induced transcripts. Transcripts displaying the largest inceptin-elicited fold changes included transcripts encoding terpene synthases (TPSs) and peroxidases (POXs) that correspond with induced volatile production and increased POX activity in cowpea. Characterization of inceptin-elicited cowpea defenses via heterologous expression in Nicotiana benthamiana demonstrated that specific cowpea TPSs and POXs were able to confer terpene emission and the reduced growth of beet armyworm (Spodoptera exigua) herbivores, respectively. Collectively, our present findings in cowpea support a model where HAMP elicitation both amplifies concurrent wound responses and specifically contributes to the activation of selective outputs associated with direct and indirect antiherbivore defenses.

Carbon-capture and storage is considered to be a potential mitigation option for climate change. However, accidental leaks of CO2 can occur, resulting in changes in ocean chemistry such as acidification and metal mobilization. Laboratory experiments were performed to provide data on the effects of CO2-related acidification on the chemical fractionation of metal(loid)s in marine-contaminated sediments using sequential extraction procedures. The results showed that sediments from Huelva estuary registered concentrations of arsenic, copper, lead, and zinc that surpass the probable biological effect level established by international protocols. Zinc had the greatest proportion in the most mobile fraction of the sediment. Metals in this fraction represent an environmental risk because they are weakly bound to sediment, and therefore more likely to migrate to the water column. Indeed, the concentration of this metal was lower in the most acidified scenarios when compared to control pH, indicating probable zinc mobilization from the sediment to the seawater.

To evaluate the effects related to the combination of potential future changes in pH, temperature and salinity on microalgae, a laboratory experiment was performed using the marine diatom Phaeodactylum tricornutum. Populations of this species were exposed during 48 h to a three-factor experimental design (3 x 2 x 2) with two artificial pH values (6, 7.4), two levels of temperature (23 degrees C, 28 degrees C), two levels of salinity (34 psu, 40 psu) and a control (pH 8, Temp 23 degrees C, Sal 34 psu). The effects on growth, cell viability, metabolic activity, and inherent cell properties (size, complexity and autofluorescence) of P. tricornutum were studied using flow cytometry. The results showed adverse effects on cultures exposed to pH 6 and high temperature and salinity, being the inherent cell properties themost sensitive response. Also, linked effects of these parameters resulted on cell viability and cell size decrease and an increase of cell autofluorescence. The conclusions obtained from this work are useful to address the potential effects of climate change (in terms of changes on pH, salinity and temperature) in microalgae. (C) 2018 Elsevier B.V. All rights reserved.

We report Na-23 nuclear magnetic resonance (NMR) measurements of the Mott insulator with strong spin- orbit interaction Ba2NaOsO6 as a function of temperature in different magnetic fields ranging from 7 T to 29 T. The measurements, intended to concurrently probe spin and orbital/lattice degrees of freedom, are an extension of our work at lower fields reported in Lu et al. (2017) [1]. We have identified clear quantitative NMR signatures that display the appearance of a canted ferromagnetic phase, which is preceded by local point symmetry breaking. We have compiled the field temperature phase diagram extending up to 29 T. We find that the broken local point symmetry phase extends over a wider temperature range as magnetic field increases.