Several lines of evidence suggest that the Milky Way underwent a major merger at z similar to 2 with the Gaia-Sausage-Enceladus (GSE) galaxy. Here we use H3 Survey data to argue that GSE entered the Galaxy on a retrograde orbit based on a population of highly retrograde stars with chemistry similar to the largely radial GSE debris. We present the first tailored N-body simulations of the merger. From a grid of approximate to 500 simulations we find that a GSE with M-* = 5 x 10(8) M-circle dot, M-DM = 2 x 10(11) M-circle dot best matches the H3 data. This simulation shows that the retrograde stars are stripped from GSE's outer disk early in the merger. Despite being selected purely on angular momenta and radial distributions, this simulation reproduces and explains the following phenomena: (i) the triaxial shape of the inner halo, whose major axis is at approximate to 35 degrees to the plane and connects GSE's apocenters; (ii) the Hercules-Aquila Cloud and the Virgo Overdensity, which arise due to apocenter pileup; and (iii) the 2 Gyr lag between the quenching of GSE and the truncation of the age distribution of the in situ halo, which tracks the lag between the first and final GSE pericenters. We make the following predictions: (i) the inner halo has a "double-break" density profile with breaks at both approximate to 15-18 kpc and 30 kpc, coincident with the GSE apocenters; and (ii) the outer halo has retrograde streams awaiting discovery at >30 kpc that contain approximate to 10% of GSE's stars. The retrograde (radial) GSE debris originates from its outer (inner) disk-exploiting this trend, we reconstruct the stellar metallicity gradient of GSE (-0.04 +/- 0.01 dex r(50)(-1)). These simulations imply that GSE delivered approximate to 20% of the Milky Way's present-day dark matter and approximate to 50% of its stellar halo.

The astrophysical origins of r-process elements remain elusive. Neutron star mergers (NSMs) and special classes of core-collapse supernovae (rCCSNe) are leading candidates. Due to these channels' distinct characteristic timescales (rCCSNe: prompt, NSMs: delayed), measuring r-process enrichment in galaxies of similar mass but differing star formation durations might prove informative. Two recently discovered disrupted dwarfs in the Milky Way's stellar halo, Kraken and Gaia-Sausage Enceladus (GSE), afford precisely this opportunity: Both have M-* approximate to 10(8) M (circle dot) but differing star formation durations of approximate to 2 Gyr and approximate to 3.6 Gyr. Here we present R approximate to 50,000 Magellan/MIKE spectroscopy for 31 stars from these systems, detecting the r-process element Eu in all stars. Stars from both systems have similar [Mg/H] approximate to -1, but Kraken has a median [Eu/Mg] approximate to -0.1 while GSE has an elevated [Eu/Mg] approximate to 0.2. With simple models, we argue NSM enrichment must be delayed by 500-1000 Myr to produce this difference. rCCSNe must also contribute, especially at early epochs, otherwise stars formed during the delay period would be Eu free. In this picture, rCCSNe account for approximate to 50% of the Eu in Kraken, approximate to 25% in GSE, and approximate to 15% in dwarfs with extended star formation durations like Sagittarius. The inferred delay time for NSM enrichment is 10x-100x longer than merger delay times from stellar population synthesis-this is not necessarily surprising because the enrichment delay includes time taken for NSM ejecta to be incorporated into subsequent generations of stars. For example, this may be due to natal kicks that result in r-enriched material deposited far from star-forming gas, which then takes approximate to 10(8)-10(9) yr to cool in these galaxies.

The standard picture of galaxy formation motivates the decomposition of the Milky Way into 3-4 stellar populations with distinct kinematic and elemental abundance distributions: the thin disk, thick disk, bulge, and stellar halo. To test this idea, we construct a Gaussian mixture model (GMM) for both simulated and observed stars in the solar neighborhood, using measured velocities and iron abundances (i.e., an augmented Toomre diagram) as the distributions to be decomposed. We compare results for the Gaia-APOGEE DR16 crossmatch catalog of the solar neighborhood with those from a suite of synthetic Gaia-APOGEE crossmatches constructed from FIRE-2 cosmological simulations of Milky Way mass galaxies. We find that in both the synthetic and real data, the best-fit GMM uses five independent components, some of whose properties resemble the standard populations predicted by galaxy formation theory. Two components can be identified unambiguously as the thin disk and another as the halo. However, instead of a single counterpart to the thick disk, there are three intermediate components with different age and alpha abundance distributions (although these data are not used to construct the model). We use decompositions of the synthetic data to show that the classified components indeed correspond to stars with different origins. By analogy with the simulated data, we show that our mixture model of the real Gaia-APOGEE crossmatch distinguishes the following components: (1) a classic thin disk of young stars on circular orbits (46%), (2) thin disk stars heated by interactions with satellites (22%), (3, 4) two components representing the velocity asymmetry of the alpha-enhanced thick disk (27%), and (5) a stellar halo consistent with early, massive accretion (4%).

Due to the different environments in the Milky Way's disc and halo, comparing wide binaries in the disc and halo is key to understanding wide binary formation and evolution. By using Gaia Early Data Release 3, we search for resolved wide binary companions in the H3 survey, a spectroscopic survey that has compiled similar to 150 000 spectra for thick-disc and halo stars to date. We identify 800 high-confidence (a contamination rate of 4 per cent) wide binaries and two resolved triples, with binary separations mostly between 10(3) and 10(5) au and a lowest [Fe/H] of -2.7. Based on their Galactic kinematics, 33 of them are halo wide binaries, and most of those are associated with the accreted Gaia-Sausage-Enceladus galaxy. The wide binary fraction in the thick disc decreases toward the low metallicity end, consistent with the previous findings for the thin disc. Our key finding is that the halo wide binary fraction is consistent with the thick-disc stars at a fixed [Fe/H]. There is no significant dependence of the wide binary fraction on the alpha-captured abundance. Therefore, the wide binary fraction is mainly determined by the iron abundance, not their disc or halo origin nor the alpha-captured abundance. Our results suggest that the formation environments play a major role for the wide binary fraction, instead of other processes like radial migration that only apply to disc stars.

Recent observations of the stellar halo have uncovered the debris of an ancient merger, Gaia-Sausage-Enceladus (GSE), estimated to have occurred greater than or similar to 8 Gyr ago. Follow-up studies have associated GSE with a large-scale tilt in the stellar halo that links two well-known stellar overdensities in diagonally opposing octants of the Galaxy (the Hercules-Aquila Cloud and Virgo Overdensity; HAC and VOD). In this paper, we study the plausibility of such unmixed merger debris persisting over several gigayears in the Galactic halo. We employ the simulated stellar halo from Naidu et al., which reproduces several key properties of the merger remnant, including the large-scale tilt. By integrating the orbits of these simulated stellar halo particles, we show that adoption of a spherical halo potential results in rapid phase mixing of the asymmetry. However, adopting a tilted halo potential preserves the initial asymmetry in the stellar halo for many gigayears. The asymmetry is preserved even when a realistic growing disk is added to the potential. These results suggest that HAC and VOD are long-lived structures that are associated with GSE and that the dark matter halo of the Galaxy is tilted with respect to the disk and aligned in the direction of HAC-VOD. Such halo-disk misalignment is common in modern cosmological simulations. Lastly, we study the relationship between the local and global stellar halo in light of a tilted global halo comprised of highly radial orbits. We find that the local halo offers a dynamically biased view of the global halo due to its displacement from the Galactic center.

The zebrafish fat-free (ffr) mutation was identified in a physiological screen for genes that regulate lipid metabolism. ffr mutant larvae are morphologically indistinguishable from wild-type sibling larvae, but their absorption of fluorescent lipids is severely impaired. Through positional cloning, we have identified a causative mutation in a highly conserved and ubiquitously expressed gene within the ffr locus. The Ffr protein contains a Dor-1 like domain typical of oligomeric Golgi complex (COG) gene, cog8. Golgi complex ultrastructure is disrupted in the ffr digestive tract. Consistent with a possible role in COG-mediated Golgi function, wild-type Ffr-GFP and COG8-mRFIP fusion proteins partially colocalize in zebrafish blastomeres. Enterocyte retention of an endosomal lipid marker in ffr larvae support the idea that altered vesicle trafficking contributes to the ffr mutant defect. These data indicate that ffr is required for both Golgi structure and vesicular trafficking, and ultimately lipid transport.

Background. Understanding the functional role(s) of the more than 20,000 proteins of the vertebrate genome is a major next step in the post-genome era. The approximately 4,000 co-translationally translocated (CTT) proteins - representing the vertebrate secretome - are important for such vertebrate-critical processes as organogenesis. However, the role(s) for most of these genes is currently unknown. Results. We identified 585 putative full-length zebrafish CTT proteins using cross-species genomic and EST-based comparative sequence analyses. We further investigated 150 of these genes (Figure 1) for unique function using morpholino-based analysis in zebrafish embryos. 12% of the CTT protein-deficient embryos resulted in specific developmental defects, a notably higher rate of gene function annotation than the 2%-3% estimate from random gene mutagenesis studies. Conclusion(s). This initial collection includes novel genes required for the development of vascular, hematopoietic, pigmentation, and craniofacial tissues, as well as lipid metabolism, and organogenesis. This study provides a framework utilizing zebrafish for the systematic assignment of biological function in a vertebrate genome.

Using a spotted 65-mer oligonucleotide microarray, we have characterized the developmental expression profile from mid-gastrulation (75% epiboly) to 5 days post-fertilization (dpf) for >16,000 unique transcripts in the zebrafish genome. Microarray profiling data sets are often immense, and one challenge is validating the results and prioritizing genes for further study. The purpose of the current study was to address such issues, as well as to generate a publicly available resource for investigators to examine the developmental expression profile of any of the over 16,000 zebrafish genes on the array. On the chips, there are 16,459 printed spots corresponding to 16,288 unique transcripts and 172 beta-actin (AF025305) spots spatially distributed throughout the chip as a positive control. We have collected 55 microarray gene expression profiling results from various zebrafish laboratories and created a Per1/CGI-based software tool (http:Hserine.umdnj.edu/similar to ouyangmi/cgi-bin/zebrafish/profile.htm) for researchers to look for the expression patterns of their gene of interest. Users can search for their genes of interest by entering the accession numbers or the nucleotide sequences and the expression profiling will be reported in the form of expression intensities versus time-course graphical displays. In order to validate this web tool, we compared 74 genes' expression results between our web tool and the in situ hybridization results from Thisse et al. [Thisse, B., Heyer, V., Lux, A., Alunni, A., Degrave, A., Seiliez, L, Kirchner, J., Parkhill, J.-P., Thisse, C., 2004. Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening. Meth. Cell. Biol. 77, 505-519] as well as those reported by Mathavan et al. [Mathavan, S., Lee, S.G., mark, A., Miller, L.D., Murthy, K.R., Tong, Y., Wu, Y.L., Lam, S.H., Yang, H., Ruan, Y., Korzh, V., Gong, Z., Liu, E.T., Lufkin, T., 2005. Transcriptome analysis of zebrafish embryogenesis using microarrays. PLoS Genet. 1, 260-276]. The comparison indicates that our microarray-derived expression patterns are 80% and 75% in agreement with the in situ database (Thisse et al., 2004) and previously published microarray data (Mathavan et al., 2005), respectively. Those genes that conflict between our web tool and the in situ database either have high sequence similarity with other genes or the in situ probes are not reliable. Among those genes that disagree between our web tool and those reported by Mathavan et al. (2005), 93% of the genes are in agreement between our web tool and the in situ database, indicating our web tool results are quite reliable. Thus, this resource provides a user-friendly web based platform for researchers to determine the developmental profile of their gene of interest and to prioritize genes identified in microarray analyses by their developmental expression profile. (c) 2007 Elsevier B.V. All rights reserved.