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  • Nicholas Wong

Nicholas
Wong

Developmental Biology & Human Health Symbiosis Model System Development

I am a 3rd year PhD student at Johns Hopkins and Carnegie Institute with the Ludington lab. I am passionate about applying interdisciplinary studies to tackle complex ecological issues. I did my undergraduate and masters in microbiology and mathematics at UCSD and Scripps Institute of Oceanography to pursue a career in microbial oceanography. I'm living the dream, studying the biological and genetic mechanisms that drive algal blooms in relation to their physical environmental conditions, and how anthropogenic influences can increase the severity and frequency of blooms. I build custom robots to grow these algae in a variety of environmentally relevant conditions. These robots help me perform genetic screens to identify a potential essential gene set that facilitate blooms. We aim to develop an encompassing model linking biological phenomena to environmental stimulus for algae to improve prediction models for blooms.

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Graduate Student
Baltimore, MD

  • Biosphere Sciences & Engineering
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Abstract
An international project was successfully completed which involved two major undertakings: (1) a round-robin to demonstrate the viability of the selected standard and ( 2) the certification of the lattice parameters of the SRM 1990, a Standard Reference Material(R) for single crystal diffractometer alignment. This SRM is a set of approximate to 3500 units of Cr-doped Al2O3, or ruby spheres [ (0.420.011 mole fraction % C ( expanded uncertainty)]. The round-robin consisted of determination of lattice parameters of a pair of crystals: the ruby sphere as a standard, and a zeolite reference to serve as an unknown. Fifty pairs of crystals were dispatched from Hauptman-Woodward Medical Research Institute to volunteers in x-ray laboratories world-wide. A total of 45 sets of data was received from 32 laboratories. The mean unit cell parameters of the ruby spheres was found to be a = 4.7608 Angstrom +/- 0.0062 Angstrom, and c = 12.9979 Angstrom +/- 0.020 Angstrom ( 95 % intervals of the laboratory means). The source of errors of outlier data was identified. The SRM project involved the certification of lattice parameters using four well-aligned single crystal diffractometers at ( Bell Laboratories) Lucent Technologies and at NRC of Canada ( 39 ruby spheres), the quantification of the Cr content using a combined microprobe and SEM/EDS technique, and the evaluation of the mosaicity of the ruby spheres using a double-crystal spectrometry method. A confirmation of the lattice parameters was also conducted using a Guinier-Hagg camera. Systematic corrections of thermal expansion and refraction corrections were applied. These rubies are rhombohedral, with space group R 3 c. The certified mean unit cell parameters are a = 4.76080 +/- 0.00029 Angstrom, and c = 12.99568 Angstrom +/- 0.00087 Angstrom ( expanded uncertainty). These certified lattice parameters fall well within the results of those obtained from the international round-robin study. The Guinier-Hagg transmission measurements on five samples of powdered rubies ( a = 4.7610 Angstrom +/- 0.0013 Angstrom, and c = 12.9954 Angstrom +/- 0.0034 Angstrom) agreed well with the values obtained from the single crystal spheres.
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