The nuclear lamina is a key structural element of the metazoan nucleus. However, the structural organization of the major proteins composing the lamina is poorly defined. Using three-dimensional structured illumination microscopy and computational image analysis, we characterized the supramolecular structures of lamin A, C, B1, and B2 in mouse embryo fibroblast nuclei. Each isoform forms a distinct fiber meshwork, with comparable physical characteristics with respect to mesh edge length, mesh face area and shape, and edge connectivity to form faces. Some differences were found in face areas among isoforms due to variation in the edge lengths and number of edges per face, suggesting that each meshwork has somewhat unique assembly characteristics. In fibroblasts null for the expression of either lamins A/C or lamin B1, the remaining lamin meshworks are altered compared with the lamin meshworks in wild-type nuclei or nuclei lacking lamin B2. Nuclei lacking LA/C exhibit slightly enlarged meshwork faces and some shape changes, whereas LB1-deficient nuclei exhibit primarily a substantial increase in face area. These studies demonstrate that individual lamin isoforms assemble into complex networks within the nuclear lamina and that A-and B-type lamins have distinct roles in maintaining the organization of the nuclear lamina.

We have surveyed the Hill sphere of Mars for irregular satellites. Our search covered nearly the entire Hill sphere, but scattered light from Mars excluded the inner few arcminutes where the satellites Phobos and Deimos reside. No new satellites were found to an apparent limiting red magnitude of 23.5, which corresponds to radii of about 0.09 km using an albedo of 0.07.

We present a deep optical survey of Uranus's Hill sphere for small satellites. The 8 m Subaru Telescope was used to survey about 3.5 square degrees with a 50% detection efficiency at limiting red magnitude m(R) = 26.1. This magnitude corresponds to objects that are about 7 km in radius (assuming an albedo of 0.04). We detected ( without prior knowledge of their positions) all previously known outer satellites and discovered two new irregular satellites (S/2001 U2 and S/2003 U3). The two inner satellites Titania and Oberon were also detected. One of the newly discovered bodies (S/2003 U3) is the first known irregular prograde satellite of the planet. The population, size distribution, and orbital parameters of Uranus's irregular satellites are remarkably similar to those of the irregular satellites of gas giant Jupiter. Both have shallow size distributions (power-law indices q similar to 2 for radii larger than 7 km) with no correlation between the sizes of the satellites and their orbital parameters. However, unlike those of Jupiter, Uranus's irregular satellites do not appear to occupy tight, distinct dynamical groups in semimajor-axis versus inclination phase space. Two groupings in semimajor-axis versus eccentricity phase space appear to be statistically significant.

A detailed description of the Halley-type Comet C/2001 OG(108) (LONEOS) has been derived from visible, near-infrared, and mid-infrared observations obtained in October and November 2001. These data represent the first high-quality ground-based observations of a bare Halley-type comet nucleus and provide the best characterization of a Halley-type comet other than 1P/Halley itself. Analysis of time series photometry suggests that the nucleus has a rotation period of 57.2 +/- 0.5 h with a minimum nuclear axial ratio of 1.3, a phase-darkening slope parameter G of -0.01 +/- 0.10, and an estimated H = 13.05 +/- 0.10. The rotation period of C/2001 OG(108) is one of the longest observed among comet nuclei. The V-R color index for this object is measured to be 0.461 +/- 0.02, which is virtually identical to that of other cometary nuclei and other possible extinct comet candidates. Measurements of the comet's thermal emission constrain the projected elliptical nuclear radii to be 9.6 +/- 1.0 km and 7.4 +/- 1.0 km, which makes C/2001 OG(108) one of the larger cometary nuclei known. The derived geometric albedo in V-band of 0.040 +/- 0.010 is typical for comet nuclei. Visible-wavelength spectrophotometry and near-infrared spectroscopy were combined to derive the nucleus's reflectance spectrum over a 0.4 to 2.5 mu m wavelength range. These measurements represent one of the few nuclear spectra ever observed and the only known spectrum of a Halley-type comet. The spectrum of this comet nucleus is very nearly linear and shows no discernable absorption features at a 5% detection limit. The lack of any features, especially in the 0.8 to 1.0 mu m range such as are seen in the spectra of carbonaceous chondrite meteorites and many low-albedo asteroids, is consistent with the presence of anhydrous rather than hydrous silicates on the surface of this comet. None of the currently recognized meteorites in the terrestrial collections have reflectance spectra that match C/2001 OG(108). The near-infrared spectrum, the geometric albedo, and the visible spectrophotometry all indicate that C/2001 OG(108) has spectral properties analogous to the D-type, and possibly P-type asteroids. Comparison of the measured albedo and diameter of C/2001 OG(108) with those of Damocloid asteroids reveals similarities between these asteroids and this comet nucleus, a finding which supports previous dynamical arguments that Damocloid asteroids could be composed of cometary-like materials. These observations are also consistent with findings that two Jupiter-family comets may have spectral signatures indicative of D-type asteroids. C/2001 OG(108) probably represents the transition from a typical active comet to an extinct cometary nucleus, and, as a Halley-type comet, suggests that some comets originating in the Oort cloud can become extinct without disintegrating. As a near-Earth object, C/2001 OG(108) supports the suggestion that some fraction of the near-Earth asteroid population consists of extinct cometary nuclei. (c) 2005 Elsevier Inc. All rights reserved.