The habitability of early Mars's subsurface to methanogenic microorganisms was assessed using a planetary ecosystem model that couples a subsurface biosphere to the atmospheric chemistry and climate. Mars's subsurface was initially likely habitable, but the biosphere would have cooled the planet down, potentially compromising its long-term habitability.

Non-mammalian model organisms have been essential for our understanding of the mechanisms that control development, disease, and physiology, but they are underutilized in pharmacological and toxicological phenotypic screening assays due to their low throughput in comparison with cell-based screens. To increase the utility of using Drosophila melanogaster in screening, we designed the Whole Animal Feeding FLat (WAFFL), a novel, flexible, and complete system for feeding, monitoring, and assaying flies in a high-throughput format. Our 3-D printed system is compatible with inexpensive and readily available, commercial 96-well plate consumables and equipment. Experimenters can change the diet at will during the experiment and video record for behavior analysis, enabling precise dosing, measurement of feeding, and analysis of behavior in 96-well plate format.

Elucidating enzyme-substrate relationships in posttranslational modification (PTM) networks is crucial for understanding signal transduction pathways but is technically difficult because enzyme-substrate interactions tend to be transient. Here we demonstrate that TurboID-based proximity labeling (TbPL) effectively and specifically captures the substrates of kinases and phosphatases. TbPL-mass spectrometry (TbPL-MS) identified over four hundred proximal proteins of Arabidopsis thaliana BRASSINOSTEROID-INSENSITIVE2 (BIN2), a member of the GLYCOGEN SYNTHASE KINASE 3 (GSK3) family that integrates signaling pathways controlling diverse developmental and acclimation processes. A large portion of the BIN2 proximal proteins showed BIN2-dependent phosphorylation in vivo or in vitro, suggesting that these are BIN2 substrates. Protein-protein interaction network analysis showed that the BIN2 proximal proteins include interactors of BIN2 substrates, revealing a high level of interactions among the BIN2 proximal proteins. Our proteomic analysis establishes the BIN2 signaling network and uncovers BIN2 functions in regulating key cellular processes such as transcription, RNA processing, translation initiation, vesicle trafficking, and cytoskeleton organization. We further discovered significant overlap between the GSK3 phosphorylome and the O-GlcNAcylome, suggesting an evolutionarily ancient relationship between GSK3 and the nutrient-sensing O-glycosylation pathway. Our work presents a powerful method for mapping PTM networks, a large dataset of GSK3 kinase substrates, and important insights into the signaling network that controls key cellular functions underlying plant growth and acclimation.

Although all-sky surveys have led to the discovery of dozens of young planets, little is known about their atmospheres. Here, we present multiwavelength transit data for the super-Neptune sized exoplanet, K2-33b-the youngest (similar to 10 Myr) transiting exoplanet to date. We combined photometric observations of K2-33 covering a total of 33 transits spanning >2 yr, taken from K2, MEarth, the Hubble Space Telescope (HST), and Spitzer. The transit photometry spanned from the optical to the near-infrared (0.6-4.5 mu m), enabling us to construct a transmission spectrum of the planet. We find that the optical transit depths are nearly a factor of 2 deeper than those from the near-infrared. This difference holds across multiple data sets taken over years, ruling out issues of data analysis and unconstrained systematics. Surface inhomogeneities on the young star can reproduce some of the difference, but required spot coverage fractions (>60%) are ruled out by the observed stellar spectrum (<20%). We find a better fit to the transmission spectrum using photochemical hazes, which were predicted to be strong in young, moderate-temperature, and large-radius planets like K2-33b. A tholin haze with CO as the dominant gaseous carbon carrier in the atmosphere can reasonably reproduce the data with small or no stellar surface inhomogeneities, consistent with the stellar spectrum. The HST data quality is insufficient for the detection of any molecular features. More observations would be required to fully characterize the hazes and spot properties and confirm the presence of CO suggested by current data.

Tissue stem cell niches are regulated by their mechanical environment, notably the extracellular matrix (ECM). Skeletal muscles consist of bundled myofibers for force transmission. Within this macroscopic architecture, quiescent Pax7-expressing (Pax7+) muscle stem cells (MuSCs) are compressed between ECM basally and myofiber apically. Muscle injury causes MuSCs to lose apical compression from the myofiber and re-enter the cell cycle for regeneration. While ECM elasticities have been shown to affect MuSC's renewal, the significance of apical compression remains unknown. To investigate the role of apical compression, we simulate the MuSCs' in vivo mechanical environment by applying physical compression to MuSCs' apical surface. We demonstrate that compression drives activated MuSCs back to a quiescent stem cell state, regardless of basal elasticities and chemistries. By mathematical modeling and cell tension manipulation, we conclude that low overall tension combined with high axial tension generated by compression leads to MuSCs' stemness and quiescence. Unexpectedly, we discovered that apical compression results in up-regulation of Notch downstream genes, accompanied by the increased levels of nuclear Notch1&3 in a Delta ligand (Dll) and ADAM10/17 independent manner. Our results fill a knowledge gap on the role of apical compression for MuSC fate and have implications to stem cells in other tissues.

Some microalgae, such as Chlamydomonas reinhardtii, harbor a highly flexible photosynthetic apparatus capable of using different electron acceptors, including carbon dioxide (CO2), protons, or oxygen (O2), allowing survival in diverse habitats. During anaerobic induction of photosynthesis, molecular O2 is produced at photosystem II, while at the photosystem I acceptor side, the reduction of protons into hydrogen (H2) by the plastidial [FeFe]-hydrogenases primes CO2 fixation. Although the interaction between H2 production and CO2 fixation has been studied extensively, their interplay with O2 produced by photosynthesis has not been considered. By simultaneously measuring gas exchange and chlorophyll fluorescence, we identified an O2 photoreduction mechanism that functions during anaerobic dark-to-light transitions and demonstrate that flavodiiron proteins (Flvs) are the major players involved in light-dependent O2 uptake. We further show that Flv-mediated O2 uptake is critical for the rapid induction of CO2 fixation but is not involved in the creation of the micro-oxic niches proposed previously to protect the [FeFe]-hydrogenase from O2 By studying a mutant lacking both hydrogenases (HYDA1 and HYDA2) and both Flvs (FLVA and FLVB), we show that the induction of photosynthesis is strongly delayed in the absence of both sets of proteins. Based on these data, we propose that Flvs are involved in an important intracellular O2 recycling process, which acts as a relay between H2 production and CO2 fixation.

Some microalgae, such as Chlamydomonas reinhardtii, harbor a highly flexible photosynthetic apparatus capable of using different electron acceptors, including carbon dioxide (CO2), protons, or oxygen (O-2), allowing survival in diverse habitats. During anaerobic induction of photosynthesis, molecular O-2 is produced at photosystem II, while at the photosystem I acceptor side, the reduction of protons into hydrogen (H-2) by the plastidial [FeFe]-hydrogenases primes CO2 fixation. Although the interaction between H-2 production and CO2 fixation has been studied extensively, their interplay with O-2 produced by photosynthesis has not been considered. By simultaneously measuring gas exchange and chlorophyll fluorescence, we identified an O-2 photoreduction mechanism that functions during anaerobic dark-to-light transitions and demonstrate that flavodiiron proteins (Flvs) are the major players involved in light-dependent O-2 uptake. We further show that Flv-mediated O-2 uptake is critical for the rapid induction of CO2 fixation but is not involved in the creation of the micro-oxic niches proposed previously to protect the [FeFe]-hydrogenase from O-2. By studying a mutant lacking both hydrogenases (HYDA1 and HYDA2) and both Flvs (FLVA and FLVB), we show that the induction of photosynthesis is strongly delayed in the absence of both sets of proteins. Based on these data, we propose that Flvs are involved in an important intracellular O-2 recycling process, which acts as a relay between H-2 production and CO2 fixation.

We present the first empirical constraints on the turbulent velocity field of the diffuse circumgalactic medium around four luminous quasi-stellar objects (QSOs) at z approximate to 0.5-1.1. Spatially extended nebulae of approximate to 50-100 physical kpc in diameter centred on the QSOs are revealed in [O II] lambda lambda 3727, 3729 and/or [O III] lambda 5008 emission lines in integral field spectroscopic observations obtained using Multi-Unit Spectroscopic Explorer on the Very Large Telescope. We measure the second- and third-order velocity structure functions (VSFs) over a range of scales, from less than or similar to 5 kpc to approximate to 20-50 kpc, to quantify the turbulent energy transfer between different scales in these nebulae. While no constraints on the energy injection and dissipation scales can be obtained from the current data, we show that robust constraints on the power-law slope of the VSFs can be determined after accounting for the effects of atmospheric seeing, spatial smoothing, and large-scale bulk flows. Out of the four QSO nebulae studied, one exhibits VSFs in spectacular agreement with the Kolmogorov law, expected for isotropic, homogeneous, and incompressible turbulent flows. The other three fields exhibit a shallower decline in the VSFs from large to small scales. However, with a limited dynamic range in the spatial scales in seeing-limited data, no constraints can be obtained for the VSF slopes of these three nebulae. For the QSO nebula consistent with the Kolmogorov law, we determine a turbulence energy cascade rate of approximate to 0.2 cm(2) s(-3). We discuss the implication of the observed VSFs in the context of QSO feeding and feedback in the circumgalactic medium.