The explosion of RNA-Seq data has enabled the identification of expressed genes without relying on gene models with biases toward open reading frames, allowing the identification of many more long noncoding RNAs (lncRNAs) in eukaryotes. Various tissue enrichment strategies and deep sequencing have also enabled the identification of an extensive list of genes expressed in maize gametophytes, tissues that are intractable to both traditional genetic and gene expression analyses. However, the function of very few genes from the lncRNA and gametophyte sets (or from their intersection) has been tested. Methods for isolating and identifying lncRNAs from gametophyte samples of maize are described here. This method is transferable to any maize gametophyte mutant enabling the development of gene networks involving both protein-coding genes and lncRNAs. Additionally, these methods can be adapted to apply to other grass model systems to test for evolutionary conservation of lncRNA expression patterns.

Author summary

The recent detection of a repeating fast radio burst (FRB) in an old globular cluster in M81 challenges traditional FRB formation mechanisms based on the magnetic activity of young neutron stars formed in core-collapse supernovae. Furthermore, the detection of this repeater in such a nearby galaxy implies a high local universe rate of similar events in globular clusters. Building off the properties inferred from the M81 FRB, we predict the number of FRB sources in nearby (d? 20 Mpc) galaxies with large globular cluster systems known. Incorporating the uncertain burst energy distribution, we estimate the rate of bursts detectable in these galaxies by radio instruments such as FAST and MeerKat. Of all local galaxies, we find M87 is the best candidate for FRB detections. We predict that M87's globular cluster system contains 0(10) FRB sources at present and that a dedicated radio survey (by either FAST or MeerKat) of 0(10) hr has a 90% probability of detecting a globular cluster FRB in M87. The detection of even a handful of additional globular cluster FRBs would provide invaluable constraints on FRB mechanisms and population properties. Previous studies have demonstrated young neutron stars formed following the collapse of dynamically formed massive white dwarf binary mergers may provide the most natural mechanism for these bursts. We explore the white dwarf merger scenario using a suite of N-body cluster models, focusing in particular on such mergers in M87's clusters. We describe a number of outstanding features of this scenario that in principle may be testable with an ensemble of observed FRBs in nearby globular clusters.

We compare mid-infrared (mid-IR), extinction-corrected H alpha, and CO (2-1) emission at 70-160 pc resolution in the first four PHANGS-JWST targets. We report correlation strengths, intensity ratios, and power-law fits relating emission in JWST's F770W, F1000W, F1130W, and F2100W bands to CO and H alpha. At these scales, CO and H alpha each correlate strongly with mid-IR emission, and these correlations are each stronger than the one relating CO to H alpha emission. This reflects that mid-IR emission simultaneously acts as a dust column density tracer, leading to a good match with the molecular-gas-tracing CO, and as a heating tracer, leading to a good match with the H alpha. By combining mid-IR, CO, and H alpha at scales where the overall correlation between cold gas and star formation begins to break down, we are able to separate these two effects. We model the mid-IR above I ( nu ) = 0.5 MJy sr(-1) at F770W, a cut designed to select regions where the molecular gas dominates the interstellar medium (ISM) mass. This bright emission can be described to first order by a model that combines a CO-tracing component and an H alpha-tracing component. The best-fitting models imply that similar to 50% of the mid-IR flux arises from molecular gas heated by the diffuse interstellar radiation field, with the remaining similar to 50% associated with bright, dusty star-forming regions. We discuss differences between the F770W, F1000W, and F1130W bands and the continuum-dominated F2100W band and suggest next steps for using the mid-IR as an ISM tracer.

JWST/Mid-Infrared Instrument imaging of the nearby galaxies IC 5332, NGC 628, NGC 1365, and NGC 7496 from PHANGS reveals a richness of gas structures that in each case form a quasi-regular network of interconnected filaments, shells, and voids. We examine whether this multiscale network of structure is consistent with the fragmentation of the gas disk through gravitational instability. We use FilFinder to detect the web of filamentary features in each galaxy and determine their characteristic radial and azimuthal spacings. These spacings are then compared to estimates of the most Toomre-unstable length (a few kiloparsecs), the turbulent Jeans length (a few hundred parsecs), and the disk scale height (tens of parsecs) reconstructed using PHANGS-Atacama Large Millimeter/submillimeter Array observations of the molecular gas as a dynamical tracer. Our analysis of the four galaxies targeted in this work indicates that Jeans-scale structure is pervasive. Future work will be essential for determining how the structure observed in gas disks impacts not only the rate and location of star formation but also how stellar feedback interacts positively or negatively with the surrounding multiphase gas reservoir.

We present a high-resolution view of bubbles within the Phantom Galaxy (NGC 628), a nearby (similar to 10 Mpc), star-forming (similar to 2 M (circle dot) yr(-1)), face-on (i similar to 9 degrees) grand-design spiral galaxy. With new data obtained as part of the Physics at High Angular resolution in Nearby GalaxieS (PHANGS)-JWST treasury program, we perform a detailed case study of two regions of interest, one of which contains the largest and most prominent bubble in the galaxy (the Phantom Void, over 1 kpc in diameter), and the other being a smaller region that may be the precursor to such a large bubble (the Precursor Phantom Void). When comparing to matched-resolution H alpha observations from the Hubble Space Telescope, we see that the ionized gas is brightest in the shells of both bubbles, and is coincident with the youngest (similar to 1 Myr) and most massive (similar to 10(5) M (circle dot)) stellar associations. We also find an older generation (similar to 20 Myr) of stellar associations is present within the bubble of the Phantom Void. From our kinematic analysis of the H I, H-2 (CO), and H ii gas across the Phantom Void, we infer a high expansion speed of around 15 to 50 km s(-1). The large size and high expansion speed of the Phantom Void suggest that the driving mechanism is sustained stellar feedback due to multiple mechanisms, where early feedback first cleared a bubble (as we observe now in the Precursor Phantom Void), and since then supernovae have been exploding within the cavity and have accelerated the shell. Finally, comparison to simulations shows a striking resemblance to our JWST observations, and suggests that such large-scale, stellar-feedback-driven bubbles should be common within other galaxies.

We combine archival Hubble Space Telescope and new James Webb Space Telescope imaging data covering the ultraviolet to mid-infrared regime to morphologically analyze the nuclear star cluster (NSC) of NGC 628, a grand-design spiral galaxy. The cluster is located in a 200 pc x 400 pc cavity lacking both dust and gas. We find roughly constant values for the effective radius (r(eff) similar to 5 pc) and ellipticity (is an element of similar to 0.05), while the Sersic index (n) and position angle (PA) drop from n similar to 3 to similar to 2 and PA similar to 130 degrees to 90 degrees, respectively. In the mid-infrared, r(eff)similar to 12 pc, is an element of similar to 0.4, and n similar to 1-1.5, with the same PA similar to 90 degrees. The NSC has a stellar mass of log(10) (M(sic)(nsc) /M-circle dot)= 7.06 +/- 0.31, as derived through B -V, confirmed when using multiwavelength data, and in agreement with the literature value. Fitting the spectral energy distribution (SED), excluding the mid-infrared data, yields a main stellar population age of (8 +/- 3) Gyr with a metallicity of Z= 0.012 +/- 0.006. There is no indication of any significant star formation over the last few gigayears. Whether gas and dust were dynamically kept out or evacuated from the central cavity remains unclear. The best fit suggests an excess of flux in the mid-infrared bands, with further indications that the center of the mid-infrared structure is displaced with respect to the optical center of the NSC. We discuss five potential scenarios, none of them fully explaining both the observed photometry and structure.

The detection of gravitational waves from the binary neuron star merger GW170817 and electromagnetic counterparts GRB170817A and AT2017gfo kick-started the field of gravitational-wave multimessenger astronomy. The optically red to near-infrared emission ("red" component) of AT2017gfo was readily explained as produced by the decay of newly created nuclei produced by rapid neutron capture (a kilonova). However, the ultraviolet to optically blue emission ("blue" component) that was dominant at early times (up to 1.5 days) received no consensus regarding its driving physics. Among many explanations, two leading contenders are kilonova radiation from a lanthanide-poor ejecta component and shock interaction (cocoon emission). In this work, we simulate AT2017gfo-like light curves and perform a Bayesian analysis to study whether an ultraviolet satellite capable of rapid gravitational-wave follow-up, could distinguish between physical processes driving the early "blue" component. We find that ultraviolet data starting at 1.2 hr distinguishes the two early radiation models up to 160 Mpc, implying that an ultraviolet mission like Dorado would significantly contribute to insights into the driving emission physics of the postmerger system. While the same ultraviolet data and optical data starting at 12 hr have limited ability to constrain model parameters separately, the combination of the two unlocks tight constraints for all but one parameter of the kilonova model up to 160 Mpc. We further find that a Dorado-like ultraviolet satellite can distinguish the early radiation models up to at least 130 (60) Mpc if data collection starts within 3.2 (5.2) hr for AT2017gfo-like light curves.

The inner solar system's modern orbital architecture provides inferences into the epoch of terrestrial planet formation; a -100 Myr time period of planet growth via collisions with planetesimals and other proto-planets. While classic numerical simulations of this scenario adequately reproduced the correct number of terrestrial worlds, their semi-major axes and approximate formation timescales, they struggled to replicate the Earth- Mars and Venus-Mercury mass ratios (-9 and 15, respectively). In a series of past independent investigations, we demonstrated that Mars' mass is possibly the result of Jupiter and Saturn's early orbital evolution, while Mercury's diminutive size might be the consequence of a primordial mass deficit in the region (potentially the result of the growing Earth's early outward migration). Here, we combine these ideas in a single modeled scenario designed to simultaneously reproduce the formation of all four terrestrial planets and the modern orbits of the giant planets in broad strokes. By evaluating our Mercury analogs' core mass fractions, masses, and orbital offsets from Venus, we favor a scenario where Mercury forms through a series of violent erosive collisions between a number of -Mercury-mass embryos in the inner part of the terrestrial disk. We also compare cases where the gas giants begin the simulation locked in a compact 3:2 resonant configuration to a more relaxed 2:1 orientation and find the former to be more successful. In 2:1 cases, the entire Mercury-forming region is often depleted due to strong sweeping secular resonances that also tend to overly excite the orbits of Earth and Venus as they grow. While our model is quite successful at replicating Mercury's massive core and dynamically isolated orbit, the planets' low mass remains extremely challenging to match. Indeed, the majority of our Mercury analogs have masses that are 2-4 times that of the real planet. Finally, we discuss the merits and drawbacks of alternative evolutionary scenarios and initial disk conditions (specifically a narrow annulus of material between 0.7-1.0 au). We argue that the results of our N-body accretion models are not sufficient to break degeneracies between these different models, and implore future studies to apply further cosmochemical and dynamical constraints on terrestrial planet formation models.