Lineage-specific stem cells are critical for the production and maintenance of specific cell types and tissues in multicellular organisms. In Arabidopsis, the initiation and proliferation of stomatal lineage cells is controlled by the basic helix-loop-helix transcription factor SPEECHLESS (SPCH). SPCH-driven asymmetric and self-renewing divisions allow flexibility in stomatal production and overall organ growth. How SPCH directs stomatal lineage cell behaviors, however, is unclear.
In my talk, I will introduce an improved chromatin immunoprecipitation (ChIP) assay that I developed for studying rare transcription regulators and discuss results on the genome-wide profiling of SPCH targets, which reveals the broad influence of SPCH in specifying an essential stem cell lineage.
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