Join Maria Pachiadaki from the Woods Hole Oceanographic Institute as she presents Distribution and ecophysiology of marine microorganisms: Unlocking the mysteries of uncultivated microbial lineages.
Abstract:
Marine microbial communities play a key role in all major biogeochemical cycles on Earth. Detection of microbial metabolic pathways and their expression in environmental samples is crucial to the understanding the ecology of microbial communities, including how they influence the chemistry of their environment, mediate interactions with other organisms, and are shaped by the environment. In the past two decades, the scientific community has advanced its capability to gather and sequence genomic and transcriptomic molecules from microorganisms, while, recently, single cell genomics has emerged as a powerful complement to cultivation and metagenomics, by providing genomic information from individual, uncultured cells. Here, I present how large omic datasets can be used to form hypotheses about the key microbial players in major biogeochemical processes, the predominant pathways used, and the expression of genes associated with those pathways. This approach provides critical information for designing targeted experiments to measure rates of microbial activities in the field.
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