Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

Grossmann, Guido; Meier, Matthias; Cartwright, Heather N.; Sosso, Davide; Quake, Stephen R.; Ehrhardt, David W.; Frommer, Wolf B.
2012
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
DOI
10.3791/4290
The root functions as the physical anchor of the plant and is the organ responsible for uptake of water and mineral nutrients such as nitrogen, phosphorus, sulfate and trace elements that plants acquire from the soil. If we want to develop sustainable approaches to producing high crop yield, we need to better understand how the root develops, takes up a wide spectrum of nutrients, and interacts with symbiotic and pathogenic organisms. To accomplish these goals, we need to be able to explore roots in microscopic detail over time periods ranging from minutes to days.